A first insight on the population structure of Mycobacterium tuberculosis complex as studied by spoligotyping and MIRU-VNTRs in Santiago, Chile

Autor: Paulina Meza, David Couvin, Nalin Rastogi, Marcela Cifuentes, Patricia García, Carlos Pena, María Elvira Balcells
Přispěvatelé: Infectious Diseases Department, School of Medicine, Pontificia Universidad Católica de Chile (UC), Microbiology Laboratory, Clinical Laboratory Department, School of Medicine, Respiratory Division and Microbiology Laboratory, Hospital San Borja Arriarán, Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), This work was supported by Chilean National Funding Agency CONICYT, FONDECYT GRANT N°1130600 (http://www.conicyt. cl/fondecyt/)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Adult
Male
Genotyping
Tuberculosis
Adolescent
Genotyping Techniques
Phylogeographical analysis
Prevalence
lcsh:Medicine
MDR-TB
Minisatellite Repeats
MIRU-VNTRs
Mycobacterium tuberculosis
Young Adult
medicine
Humans
Chile
Child
lcsh:Science
Aged
Aged
80 and over
Spoligotyping
Molecular Epidemiology
Multidisciplinary
Molecular epidemiology
biology
lcsh:R
Extensively drug-resistant tuberculosis
Genetic Variation
Middle Aged
biology.organism_classification
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Interspersed Repetitive Sequences
Phylogeography
Mycobacterium tuberculosis complex
Child
Preschool
Latin-America
Multilocus sequence typing
Female
lcsh:Q
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Demography
Research Article
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2015, 10 (2), pp.e0118007. ⟨10.1371/journal.pone.0118007⟩
PLoS ONE, Vol 10, Iss 2, p e0118007 (2015)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0118007⟩
Popis: International audience; Tuberculosis (TB) remains a significant public health problem worldwide, but the ecology of the prevalent mycobacterial strains, and their transmission, can vary depending on country and region. Chile is a country with low incidence of TB, that has a geographically isolated location in relation to the rest of South American countries due to the Andes Mountains, but recent migration from neighboring countries has changed this situation. We aimed to assess the genotypic diversity of Mycobacterium tuberculosis complex (MTBC) strains in Santiago, Chile, and compare with reports from other Latin-American countries. We analyzed MTBC isolates from pulmonary tuberculosis cases collected between years 2008 and 2013 in Central Santiago, using two genotyping methods: spoligotyping and 12-loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTRs). Data obtained were analyzed and compared to the SITVIT2 database. Mean age of the patients was 47.5 years and 61% were male; 11.6% were migrants. Of 103 strains (1 isolate/patient) included, there were 56 distinct spoligotype patterns. Of these, 16 strains (15.5%) corresponded to orphan strains in the SITVIT2 database, not previously reported. Latin American and Mediterranean (LAM) (34%) and T (33%) lineages were the most prevalent strains, followed by Haarlem lineage (16.5%). Beijing family was scarcely represented with only two cases (1.9%), one of them isolated from a Peruvian migrant. The most frequent clustered spoligotypes were SIT33/LAM3 (10.7%), SIT53/T1 (8.7%), SIT50/H3 (7.8%), and SIT37/T3 (6.8%). We conclude that LAM and T genotypes are the most prevalent genotypes of MTBC in Santiago, Chile, and together correspond to almost two thirds of analyzed strains, which is similar to strain distribution reported from other countries of Latin America. Nevertheless, the high proportion of SIT37/T3, which was rarely found in other Latin American countries, may underline a specific history or demographics of Chile related to probable human migrations and evolutions.
Databáze: OpenAIRE
Externí odkaz: