Stem cell topography splits growth and homeostatic functions in the fish gill

Autor: Kiyoshi Naruse, Elizabeth Mayela Ambrosio, Anna Marciniak-Czochra, Diana-Patricia Danciu, Lorena Buono, Juan Ramón Martínez-Morales, Julian Stolper, Lazaro Centanin, David A. Elliott
Přispěvatelé: German Research Foundation, Fundación Ramón Areces, University of Melbourne
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: eLife
eLife, Vol 8 (2019)
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 2050-084X
Popis: While lower vertebrates contain adult stem cells (aSCs) that maintain homeostasis and drive un-exhaustive organismal growth, mammalian aSCs display mainly the homeostatic function. Here, we use lineage analysis in the medaka fish gill to address aSCs and report separate stem cell populations for homeostasis and growth. These aSCs are fate-restricted during the entire post-embryonic life and even during re-generation paradigms. We use chimeric animals to demonstrate that p53 mediates growth coordination among fate-restricted aSCs, suggesting a hierarchical organisation among lineages in composite organs like the fish gill. Homeostatic and growth aSCs are clonal but differ in their topology; modifications in tissue architecture can convert the homeostatic zone into a growth zone, indicating a leading role for the physical niche defining stem cell output. We hypothesise that physical niches are main players to restrict aSCs to a homeostatic function in animals with fixed adult size.
This work has been funded by the Deutsche Forschungsgemeinschaft (German Research Foundation, DFG) via the Collaborative Research Centre SFB873 (subproject A11 to LC and B08 to AMC). LB gratefully acknowledges Ramon Areces Foundation’s support, D-PD the Research Training Group (Landesgraduiertenkolleg) “Mathematical Modeling for the Quantitative Biosciences” and Heidelberg Graduate School (HGS MathComp) and LC acknowledges support from the DAAD. JS is the recipient of a Melbourne Research Scholarship from the University of Melbourne, Australia.
Databáze: OpenAIRE