Clinical Evaluation of Affirm VPIII in the Detection and Identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida Species in Vaginitis/Vaginosis
| Autor: | Justin D. Wright, Thomas E. Davis, Haywood L. Brown, Lori T. Jasper, DeAnna Fuller |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Indiana
medicine.medical_specialty Vaginal Diseases education Trichomonas Dermatology medicine.disease_cause Sensitivity and Specificity lcsh:Gynecology and obstetrics lcsh:Infectious and parasitic diseases Vaginal disease Trichomonas Vaginitis Predictive Value of Tests Gardnerella Trichomonas vaginalis medicine Animals Humans Gardnerella vaginalis lcsh:RC109-216 Candidiasis Vulvovaginal lcsh:RG1-991 Candida Vaginitis Vaginal Smears Gynecology biology business.industry Obstetrics and Gynecology Vaginosis Bacterial biology.organism_classification medicine.disease humanities Infectious Diseases Female Reagent Kits Diagnostic business Clinical evaluation Research Article |
| Zdroj: | Infectious Diseases in Obstetrics and Gynecology, Vol 12, Iss 1, Pp 17-21 (2004) Infectious Diseases in Obstetrics and Gynecology |
| ISSN: | 1098-0997 1064-7449 |
| DOI: | 10.1080/1064744042000210375 |
| Popis: | OBJECTIVE: To compare the Affirm VPIII Microbial Identification Test for detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis to clinical and microscopic criteria commonly used to diagnose vaginitis. METHODS: Women that were symptomatic for vaginitis/vaginosis and asymptomatic women being seen for routine obstetric or gynecological care were included in this study. Women treated with antibiotics or antifungals within one week or women who had douched within 24 hours were excluded. Two vaginal swab specimens were simultaneously obtained from each patient, one swab was placed in sterile physiological saline for immediate microscopic wet mount examination and KOH testing. The other swab was placed in the Affirm collection tube for Affirm VPIII testing based on previously demonstrated methods. RESULTS: The Affirm assay was significantly more likely to identify Gardnerella and Candida than wet mount. 190 (45%) were positive for Gardnerella by Affirm compared to 58 (14%) by wet mount; 45 (11%) were positive for Candida by Affirm compared to 31 (7%) by wet mount; and 30 (7%) were positive for Trichomonas by Affirm compared to 23 (5%) by wet mount. Symptomatic women were significantly more likely to be positive by Affirm only (23% vs. 10%), wet mount only (3% vs. 2%) or Affirm and wet mount (15% vs. 1%). Asymptomatic women were significantly more likely to be negative for Affirm and wet mount (43% vs. 5%). CONCLUSIONS: The Affirm VPIII test is a more sensitive diagnostic test for detection and identification of symptomatic vaginitis/vaginosis than conventional clinical examination and wet mount testing. |
| Databáze: | OpenAIRE |
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