Crystallization and avoiding the problem of hemihedral twinning in crystals of Delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus
| Autor: | Chizu Kuroishi, Eiji Inagaki, Hitomi Takahashi, Tahir H. Tahirov |
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| Rok vydání: | 2005 |
| Předmět: |
Biophysics
Glutamic Acid Dehydrogenase Biochemistry Polymerase Chain Reaction Citric Acid law.invention Bacterial Proteins X-Ray Diffraction Structural Biology law Genetics Crystallization biology Chemistry Thermus thermophilus Space group Glutamic acid Condensed Matter Physics biology.organism_classification NAD Crystallography 1-Pyrroline-5-Carboxylate Dehydrogenase Crystallization Communications X-ray crystallography Crystal twinning |
| Zdroj: | Acta crystallographica. Section F, Structural biology and crystallization communications. 61(Pt 6) |
| ISSN: | 1744-3091 |
| Popis: | Delta1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 A resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 A, and diffract to 1.08 A. Crystals soaked in solution with NAD+ (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized. |
| Databáze: | OpenAIRE |
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