Silencing the activity and proliferative properties of the human EagI Potassium Channel by RNA Interference
| Autor: | Fernanda Mello de Queiroz, Arnt Suckow, Bryan R. Downie, Walter Stühmer, Claudia Weber, Luis A. Pardo |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Small interfering RNA
Time Factors Cell growth RNA Cell Biology Biology medicine.disease_cause Biochemistry Molecular biology Ether-A-Go-Go Potassium Channels Cell biology Malignant transformation RNA interference Cell Line Tumor Gene expression medicine Gene silencing Humans RNA Interference RNA Messenger Carcinogenesis Molecular Biology Cell Proliferation |
| Zdroj: | J Biol Chem |
| ISSN: | 0021-9258 |
| Popis: | EagI potassium channels are natively expressed in the mammalian brain as well as in many cancer cell lines and tumor tissues. The role of EagI in malignant transformation has been suggested by several experiments, but the lack of specific EagI inhibitors has made it difficult to examine the influence of the channel on oncogenesis and its potential as a therapeutic target. We have used short interfering RNA to test the effects of EagI reduction on the behavior of tumor cells in vitro. By generating and optimizing an EagI-specific short interfering RNA system, we were able to study the effects of EagI depletion on several cancer cell lines that endogenously express this protein. We show here that our short interfering RNA sequences act specifically on EagI, reproducibly induce a significant decrease in the proliferation of tumor cell lines, and do not trigger any observable nonspecific responses. |
| Databáze: | OpenAIRE |
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