NgoII, a restriction endonuclease from Neisseria gonorrhoeae
| Autor: | W S Riggsby, R V Miller, D J Clanton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1979 |
| Předmět: |
Biology
medicine.disease_cause Microbiology Coliphages HaeIII Restriction fragment chemistry.chemical_compound Isoschizomer medicine Magnesium Molecular Biology Base Sequence DNA Restriction Enzymes Lambda phage Hydrogen-Ion Concentration biology.organism_classification Molecular biology Enzyme assay Neisseria gonorrhoeae Isoenzymes Molecular Weight Restriction enzyme chemistry Biochemistry DNA Viral biology.protein DNA medicine.drug Research Article |
| Popis: | EndoR . NgoII, a class II restriction endonuclease isolated from Neisseria gonorrhoeae, was purified to electrophoretic homogeneity. We were able to separate it from another restriction endonuclease of N. gonorrhoeae, NgoI, by phosphocellulose chromatography. NgoII is an isoschizomer of HaeIII, a restriction endonuclease of Haemophilus aegyptius, and was found to recognize the deoxyribonucleic acid nucleotide base sequence GGCC. NgoII was able to digest phage lambda deoxyribonucleic acid over a wide pH range, with optimal activity at pH 8.5. The enzyme has an absolute requirement for Mg2+; maximal enzyme activity was observed at 1 mM Mg2+. The active enzyme has a molecular weight of 65,000 and appears to be composed of six subunits of identical molecular weight (11,000). No methylase activity could be detected in the purified enzyme preparation. |
| Databáze: | OpenAIRE |
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