Analytical applications of enzymatic growth of quantum dots
| Autor: | Laura Saa, Jose Sanchez-Lopez, Ana Virel, Valery Pavlov |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chemistry
Sulfates Organic Chemistry Cadmium sulfate Substrate (chemistry) Nanotechnology General Chemistry Photochemistry Alkaline Phosphatase Catalysis Fluorescence spectroscopy Thiophosphate chemistry.chemical_compound Thiocholine Quantum dot Acetylthiocholine Quantum Dots Acetylcholinesterase Biocatalysis Cadmium Compounds Alkaline phosphatase Hydrogen Sulfide |
| Zdroj: | Chemistry (Weinheim an der Bergstrasse, Germany). 16(21) |
| ISSN: | 1521-3765 |
| Popis: | We have developed an analytical assay to detect the enzymatic activity of acetylcholine esterase and alkaline phosphatase based on the generation of quantum dots by enzymatic products. Acetylcholine esterase converts acetylthiocholine into thiocholine. The latter enhances the rate of decomposition of sodium thiosulfate into H(2)S, which in the presence of cadmium sulfate yields CdS quantum dots showing a time dependent exponential growth, typical of autocatalytic processes. This assay was also applied to detect acetylcholine esterase inhibitors. Alkaline phosphatase hydrolyzes thiophosphate and yields H(2)S, which instantly reacts with Cd(2+) to give CdS quantum dots. The formation of CdS quantum dots in both reactions was followed by fluorescence spectroscopy and showed dependence on the concentration of enzyme and substrate. |
| Databáze: | OpenAIRE |
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