The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases
| Autor: | Jörn Walter, Thomas A. Trautner, Mario Noyer-Weidner |
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| Rok vydání: | 1990 |
| Předmět: |
DNA-Cytosine Methylases
Methyltransferase Molecular Sequence Data Restriction Mapping Biology Methylation DNA methyltransferase General Biochemistry Genetics and Molecular Biology Substrate Specificity chemistry.chemical_compound Restriction map Sequence Homology Nucleic Acid Amino Acid Sequence DNA (Cytosine-5-)-Methyltransferases Cloning Molecular Molecular Biology Peptide sequence Genetics Base Sequence General Immunology and Microbiology General Neuroscience Nucleic acid sequence Restriction enzyme chemistry Biochemistry Genes Bacterial DNA Cytosine Research Article Bacillus subtilis |
| Zdroj: | The EMBO Journal. 9:1007-1013 |
| ISSN: | 0261-4189 |
| Popis: | The Bacillus subtilis FI DNA methyltransferase (M.BsuFI) modifies the outer cytosine of the DNA sequence CCGG, causing resistance against R.BsuFI and R.MspI restriction. The M.BsuFI gene was cloned and expressed in B.subtilis and Escherichia coli. As derived from the nucleotide sequence, the M.BsuFI protein has 409 amino acids, corresponding to a molecular mass of 46,918 daltons. Including these data we have compared the nucleotide and amino acid sequences of different CCGG recognizing enzymes. These analyses showed that M.BsuFI is highly related to two other CCGG specific methyltransferases, M.MspI and M.HpaII, which were isolated from Gram-negative bacteria. Between M.BsuFI and M.MspI the sequence similarity is particularly significant in a region, which has been postulated to contain the target recognition domains (TRDs) of cytosine-specific DNA methyltransferases. Apparently M.BsuFI and M.MspI, derived from phylogenetic distant organisms, use highly conserved structural elements for the recognition of the CCGG target sequence. In contrast the very same region of M.HpaII is quite different from those of M.BsuFI and M.MspI. We attribute this difference to the different targeting of methylation within the sequence CCGG, where M.HpaII methylates the inner, M.BsuFI/M.MspI the outer cytosine. Also the CCGG recognizing TRD of the multispecific B.subtilis phage SPR Mtase is distinct from that of the host enzyme, possibly indicating different requirements for TRDs operative in mono- and multispecific enzymes. |
| Databáze: | OpenAIRE |
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