Influence of the ferric uptake regulator (Fur) protein on pathogenicity in Pectobacterium carotovorum subsp. brasiliense
| Autor: | Lucy N. Moleleki, Divine Yutefar Shyntum, Collins Kipngetich Tanui, Jacques Theron, Stefan Louis Priem |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Exopolysaccharides Mutant Glycobiology Siderophores Gene Expression lcsh:Medicine Artificial Gene Amplification and Extension Pectobacterium carotovorum Plant Science Pathology and Laboratory Medicine Polymerase Chain Reaction Biochemistry law.invention Plasmid 4-Butyrolactone law Vegetables Medicine and Health Sciences lcsh:Science Polymerase chain reaction Multidisciplinary Virulence Plant Anatomy Kanamycin Plants Mutant Strains Host-Pathogen Interactions Pathogens Potato Research Article medicine.drug Virulence Factors Iron 030106 microbiology Down-Regulation Mutagenesis (molecular biology technique) Biology Solanum Research and Analysis Methods Microbiology 03 medical and health sciences Bacterial Proteins Gene Types Polysaccharides Genetics medicine Gene Regulation Molecular Biology Techniques Molecular Biology Gene Solanum tuberosum Tubers Superoxide Dismutase lcsh:R Organisms Biology and Life Sciences Hydrogen Peroxide biology.organism_classification Repressor Proteins Oxidative Stress Mutagenesis Biofilms Mutation Regulator Genes bacteria lcsh:Q |
| Zdroj: | PLoS ONE, Vol 12, Iss 5, p e0177647 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Iron is an important nutrient for the survival and growth of many organisms. In order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. The ferric uptake regulator protein (Fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. However, to date, the role played by Fur in the biology of Pectobacterium carotovorum subsp. brasiliense (Pcb1692), an important pathogen of potatoes, has not yet been studied. To this end, we used the lambda recombineering method to generate a fur mutant strain of Pcb1692 and assessed the virulence and fitness of the mutant strain. The results showed that production of siderophores in Pcb1692Δfur increased compared to the Pcb1692 wild-type and the complemented strain Pcb1692Δfur-pfur. However, production of N-acyl homoserine lactone (AHLs), biofilm formation, exopolysaccharide (EPS) production, virulence on potato tubers and swimming motility, were all significantly decreased in Pcb1692Δfur compared to the wild-type and complemented Pcb1692Δfur-pfur strains. The Pcb1692Δfur mutant also demonstrated significant sensitivity to oxidative stress when exposed to H2O2. Consistent with phenotypic results, qRT-PCR results demonstrated that Fur down-regulates genes which encode proteins associated with: iron uptake (HasA-extracellular heme-binding protein and Ferrodoxin-AED-0004132), stress response (SodC-superoxide dismutase), plant cell wall degrading enzymes (PrtA and CelV) and motility (FlhC and MotA). We conclude that the ferric uptake regulator protein (Fur) of Pcb1692 regulates traits that are important to host-pathogens interactions. |
| Databáze: | OpenAIRE |
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