Methods for Generating Highly Magnetically Responsive Lanthanide-Chelating Phospholipid Polymolecular Assemblies
| Autor: | Erich J. Windhab, Mirjam E. Baumgartner, Pernille Qwist Reckey, Takashi Ishikawa, Joachim Kohlbrecher, Stéphane Isabettini, Simon Kuster, Peter Fischer |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Lanthanide
Chemistry Bilayer Phospholipid 02 engineering and technology Surfaces and Interfaces Model lipid bilayer 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Ion chemistry.chemical_compound Chemical engineering Electrochemistry Organic chemistry General Materials Science Chelation Extrusion 0210 nano-technology Spectroscopy Conjugate |
| Zdroj: | Langmuir, 33 (25) |
| Popis: | Mixtures of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and its lanthanide ion (Ln3+) chelating phospholipid conjugate, 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine-diethylene triaminepentaacetate (DMPE-DTPA), assemble into highly magnetically responsive polymolecular assemblies such as DMPC/DMPE-DTPA/Ln3+ (molar ratio 4:1:1) bicelles. Their geometry and magnetic alignability is enhanced by introducing cholesterol into the bilayer in DMPC/Cholesterol/DMPE-DTPA/Ln3+ (molar ratio 16:4:5:5). However, the reported fabrication procedures remain tedious and limit the generation of highly magnetically alignable species. Herein, a simplified procedure where freeze thawing cycles and extrusion are replaced by gentle heating and cooling cycles for the hydration of the dry lipid film was developed. Heating above the phase transition temperature Tm of the lipids composing the bilayer before cooling back below the Tm was essential to guarantee successful formation of the polymolecular assemblies composed o... |
| Databáze: | OpenAIRE |
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