An Adenoviral Platform for Selective Self-Assembly and Targeted Delivery of Nanoparticles
| Autor: | Larisa Pereboeva, Vaibhav Saini, S. B. Mirov, David T. Curiel, Dmitri V. Martyshkin, Guy Perkins, Hongju Wu, Anton V. Borovjagin, Mark H. Ellisman, Victoria D. Towner, Maaike Everts, Alex Perez |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
Genetic enhancement Genetic Vectors Gene Expression Metal Nanoparticles Nanoparticle Nanotechnology Computational biology Adenoviridae Cell Line Biomaterials Microscopy Electron Transmission Humans General Materials Science Vector (molecular biology) Amino acid residue Metal nanoparticles Spectrophotometry Atomic technology industry and agriculture Treatment options Genetic Therapy General Chemistry Carcinoembryonic Antigen Chemical coupling Capsid Proteins Gold Self-assembly HeLa Cells Biotechnology |
| Zdroj: | Small. 4:262-269 |
| ISSN: | 1613-6829 1613-6810 |
| DOI: | 10.1002/smll.200700403 |
| Popis: | Metallic nanoparticles (NPs) can be used for the diagnosis, imaging, and therapy of tumors and cardiovascular disease. However, targeted delivery of NPs to specific cells remains a major limitation for clinical realization of these potential treatment options. Herein, a novel strategy for the specific coupling of NPs to a targeted adenoviral (Ad) platform to deliver NPs to specific cells is defined. Genetic manipulation of the gene-therapy vector is combined with a specific chemical coupling strategy. In particular, a high-affinity interaction between a sequence of six-histidine amino acid residues genetically incorporated into Ad capsid proteins and nickel(II) nitrilotriacetic acid on the surface of gold NPs is employed. The selective self-assembly of gold NPs and Ad vectors into multifunctional platforms does not negatively affect the targeting of Ad to specific cells. This opens the possibility of using Ad vectors for targeted NP delivery, thereby providing a new type of combinatorial approach for the treatment of diseases that involves both nanotechnology and gene therapy. |
| Databáze: | OpenAIRE |
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