Rat hypothalamic GRF elicits its biologic action in GH3 cells by interaction with VIP- preferring receptor site(s)
| Autor: | Fusun N. Zeytin, Florence Reyl-Desmars, Timothy Rathbun |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class Biophysics Hypothalamus Stimulation Peptide Receptors Cell Surface Growth Hormone-Releasing Hormone Biochemistry Cell Line Pituitary Gland Anterior Internal medicine medicine Cyclic AMP Animals Humans Pituitary Neoplasms Receptor Molecular Biology chemistry.chemical_classification Cell Biology In vitro Rats Rat Pituitary Kinetics Endocrinology Mechanism of action chemistry Growth Hormone Receptors Vasoactive Intestinal Peptide Efflux medicine.symptom Vasoactive Intestinal Peptide |
| Zdroj: | Biochemical and biophysical research communications. 127(3) |
| ISSN: | 0006-291X |
| Popis: | Summary GH 3 cells can be used effectively to study the in vitro mechanism of action of GRF. In these cells, there is a time and concentration-dependent release of cAMP into the medium. Rat hypothalamic GRF, (rGRF) is 7 to 10 fold more active than human hypothalamic GRF (hGRF). VIP, a peptide which is structurally homologous to GRF, stimulates cAMP efflux in GH 3 cells, with a higher affinity than hGRF or rGRF. We propose that in contradistinction to the normal rat pituitary, the stimulation of cAMP release by GRF in GH 3 cells occurs via activation of VIP-preferring receptors and that GRF (rGRF in particular) behaves as a partial VIP agonist. |
| Databáze: | OpenAIRE |
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