The Prostaglandin Synthase 2/cyclooxygenase 2 (PTGS2/COX2) rs5277 Polymorphism Does not Influence Risk of Colorectal Cancer in an Iranian Population
| Autor: | Shohreh Almasi, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali, Mohsen Vahedi, Mahdi Montazer Haghighi, Pedram Azimzadeh, Ehsan Nazemalhosseini Mojarad, Behzad Damavand, Fatemeh Khorshidi |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology Cancer Research medicine.medical_specialty Pathology Epidemiology Colorectal cancer Iran Polymerase Chain Reaction Polymorphism Single Nucleotide Polymorphism (computer science) Internal medicine Genotype Odds Ratio medicine Humans Genetic Predisposition to Disease Genotyping Aged business.industry Carcinoma Public Health Environmental and Occupational Health Case-control study Odds ratio Middle Aged medicine.disease Logistic Models Cyclooxygenase 2 Case-Control Studies Female Gene polymorphism Restriction fragment length polymorphism Colorectal Neoplasms business Polymorphism Restriction Fragment Length |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 15:3507-3511 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2014.15.8.3507 |
| Popis: | Background: The prostaglandin-endoperoxide synthase 2 [PTGS2, commonly known as cyclooxygenase-2 (COX-2)] is an enzyme induced by proinflammatory stimuli that is often overexpressed in malignant tissue and involved in the synthesis of prostaglandins and thromboxanes, regulators of processes such as inflammation, cell proliferation, and angiogenesis, all relevant for cancer development. We investigated whether a functional genetic polymorphism, rs5277, in COX-2 may have a risk-modifying effect on sporadic colorectal cancer in an Iranian population. Materials and Methods: We conducted a case-control study on 167 patients with colorectal cancer and 197 cancer-free controls in Taleghani Hospital in Tehran, Iran, between 2007 and 2011. Peripheral blood samples of both groups were processed for DNA extraction and genotyping of the COX-2 gene polymorphism (rs5277) using PCR-RFLP. RFLP results were confirmed by direct sequencing. Logistic regression analysis was performed to calculate the adjusted odds ratio (OR) and 95% confidence interval (95% CI). Results: There was no significant difference in the distribution of COX-2 gene rs5277 polymorphism genotype and the allelic form, among CRC patients compared with the healthy control group (p: 0.867). Conclusions: Our results suggest that rs5277 polymorphism in COX2 could not be a good prognostic indicator for patients with CRC. |
| Databáze: | OpenAIRE |
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