Localization of nerve growth factor (NGF) receptors in the mitochondrial compartment: Characterization and putative role
| Autor: | Antonio Russo, Ida Perrotta, Valentina Carito, Giuseppe Genchi, Domenico Mancuso, Maria Cristina Caroleo, Attilio Pingitore, Erika Cione |
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| Rok vydání: | 2012 |
| Předmět: |
Cell Membrane Permeability
Blotting Western Biophysics Receptors Nerve Growth Factor Tropomyosin receptor kinase A Mitochondrion Kidney p38 Mitogen-Activated Protein Kinases Biochemistry Cell Line medicine Animals Humans Immunoprecipitation Receptor trkA Extracellular Signal-Regulated MAP Kinases Receptor Molecular Biology Cell Proliferation biology Podocytes Adenine nucleotide translocator Adenine Nucleotide Translocator 1 Brain Cell Compartmentation Cyclic Nucleotide Phosphodiesterases Type 4 Mitochondria Rats Cell biology Protein Transport Nerve growth factor medicine.anatomical_structure nervous system Mitochondrial permeability transition pore Organ Specificity Child Preschool Peripheral nervous system biology.protein Calcium Neurotrophin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1820:96-103 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2011.10.015 |
| Popis: | Background The neurotrophin NGF receptors trkA and p75NTR are expressed in the central and peripheral nervous system as well as in non-neuronal tissues; originally described to localize to the plasma membrane, recent studies have suggested other intracellular localizations for both NGF receptors. Scope of review In order to determine whether NGF receptors localize to the mitochondrial compartment mitochondria isolated from human kidney, rat tissues and a human podocyte as cell line before and after differentiation were used. Major conclusions Our results demonstrate that NGF receptors are localized in the mitochondrial compartment of undifferentiated human podocytes and in all tissues analyzed including rat central nervous system. In mitochondria p75NTR, but not trkA, co-immunoprecipitates with the adenine nucleotide translocator (ANT) and the phosphodiesterase 4 isoform A5 (PDE4A5). Moreover, NGF, via trkA, protects isolated mitochondria of rat brain cortex from mitochondrial permeability transition induced by Ca2+. General significance Although NGF receptors have been described as mainly citoplasmatic so far, we proved evidence of their expression at the mitochondrial level and their interaction with specific proteins. Our results demonstrating the expression of NGF receptors in the mitochondria provide new insights into the role of NGF at subcellular level, in different areas of the organism, including CNS. |
| Databáze: | OpenAIRE |
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