Abnormal lipid metabolism in a rat model of arthritis: one possible pathway
| Autor: | Neeraj Sinha, Niraj Kumar Srivastava, Shikha Sharma, Sudhir Kumar Mandal, Deepak Sharma |
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| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine medicine.medical_specialty Antioxidant Clinical chemistry medicine.medical_treatment Clinical Biochemistry Arthritis Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Synovitis Internal medicine Lactate dehydrogenase medicine Animals Molecular Biology 030203 arthritis & rheumatology Lipid metabolism Cell Biology General Medicine Lipid Metabolism medicine.disease Arthritis Experimental Lipids Rats Metabolic pathway 030104 developmental biology Endocrinology chemistry Cytokines Female lipids (amino acids peptides and proteins) |
| Zdroj: | Molecular and Cellular Biochemistry. 448:107-124 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | Collagen-induced arthritis (CIA) animal model is associated with systemic manifestations, including alteration of lipid metabolism. In the present study, one possible pathway of altered lipid metabolism is proposed. Specimens of joint tissue and plasma were collected from the CIA and control rats, and quantitative analysis of lipid components was performed by nuclear magnetic resonance (NMR) spectroscopy technique. Correlation analysis was performed between the level of lipid components and antioxidant enzymes, lactate dehydrogenase (LDH), lipid peroxidation (LP), and cytokines in joint tissue and plasma. Differentiation between the CIA and control rats was established on the basis of the quantity of lipid components in the joint tissue and plasma. Positive correlation was observed for all the enzymes vs. lipid components as well as LP vs. lipid components in plasma and joint tissue. Positive correlation was observed for enzymes in plasma and joint tissue. A negative correlation was observed in between the plasma and joint tissue with the level of lipid components. Cytokine levels were also correlated with the level of lipid components and ratios of saturated fatty acids/unsaturated fatty acids in plasma and joint tissue. Inflammatory disease activity in CIA rats with synovitis brought about a significant change in lipid metabolism. Taken together, the results of our study are delineating a possible pathway of altered lipid metabolism in the CIA rat model, thereby contributing further to an understanding of the pathophysiology of rheumatoid arthritis (RA). |
| Databáze: | OpenAIRE |
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