| Popis: |
PDF file - 128K, Two mechanistically-distinct mTOR inhibitors, rapamycin and INK-128, show differential abilities to inhibit signaling components upstream of mTORC1. SKBR3 cells were serum-starved for 40-48 h. Rapamycin (50 nM) or INK (50 nM) was added to the cells for 30 minutes prior to the additional treatment of cells with or without 1 nM HRG for 30 min. Cells were then harvested, lysed, and then lysates were analyzed by Western blotting for relative levels of phospho-mTOR (S2448), phospho-TSC2 (T1462), phopsho-AKT (T308), phospho-AKT (S473) and the corresponding total proteins. |
