Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers

Autor: Guido J. E. J. Hooiveld, Joris Hoeks, Wouter D. van Marken Lichtenbelt, Matthijs H. C. Hesselink, Sander Kersten, Roland W. J. Hangelbroek, Emmani B.M. Nascimento, Patrick Schrauwen
Přispěvatelé: RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health, Nutrition and Movement Sciences
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Acclimatization
Skeletal muscle
Transcriptome
Voeding
Metabolisme en Genomica
0302 clinical medicine
Brown adipose tissue
Myocyte
Genetics (clinical)
biology
BROWN ADIPOSE-TISSUE
Type 2 diabetes
Middle Aged
Insulin sensitivity
Metabolism and Genomics
Healthy Volunteers
Cold Temperature
medicine.anatomical_structure
030220 oncology & carcinogenesis
Metabolisme en Genomica
Nutrition
Metabolism and Genomics
Research Article
EXPRESSION
medicine.medical_specialty
lcsh:Internal medicine
lcsh:QH426-470
Cold acclimation
CAPACITY
Exercise training
03 medical and health sciences
Downregulation and upregulation
Voeding
Internal medicine
Genetics
medicine
Humans
lcsh:RC31-1245
Muscle
Skeletal
Exercise
Nutrition
VLAG
IDENTIFICATION
SET ENRICHMENT ANALYSIS
Insulin receptor
lcsh:Genetics
030104 developmental biology
Endocrinology
Diabetes Mellitus
Type 2
Gene Expression Regulation
biology.protein
GLUT4
Biomarkers
Zdroj: BMC Medical Genomics
BMC Medical Genomics 13 (2020)
BMC Medical Genomics, 13
BMC Medical Genomics, 13(1):124. BioMed Central Ltd
BMC Medical Genomics, Vol 13, Iss 1, Pp 1-11 (2020)
ISSN: 1755-8794
Popis: Background Cold acclimation and exercise training were previously shown to increase peripheral insulin sensitivity in human volunteers with type 2 diabetes. Although cold is a potent activator of brown adipose tissue, the increase in peripheral insulin sensitivity by cold is largely mediated by events occurring in skeletal muscle and at least partly involves GLUT4 translocation, as is also observed for exercise training. Methods To investigate if cold acclimation and exercise training overlap in the molecular adaptive response in skeletal muscle, we performed transcriptomics analysis on vastus lateralis muscle collected from human subjects before and after 10 days of cold acclimation, as well as before and after a 12-week exercise training intervention. Results Cold acclimation altered the expression of 756 genes (422 up, 334 down, P Conclusions Overall, our results indicate that cold acclimation and exercise training have overlapping effects on gene expression in human skeletal muscle, but strikingly these overlapping genes are designated to pathways related to tissue remodeling rather than metabolic pathways.
Databáze: OpenAIRE
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