Galactose to tagatose isomerization by the l-arabinose isomerase from Bacillus subtilis: a biorefinery approach for Gelidium sesquipedale valorisation
| Autor: | Carla Cristina Marques de Oliveira, Lucília Domingues, Aloia Romaní, Sara Ferreira, Cristina M. R. Rocha, Sara Isabel Leite Baptista |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Arabinose Bioconversion Biorefinery approach Red macroalgae L-arabinose isomerase 7. Clean energy 01 natural sciences Hydrolysate 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Gelidium sesquipedale Food science Tagatose 030304 developmental biology 0303 health sciences l-arabinose isomerase Science & Technology 9. Industry and infrastructure Rare sugar chemistry 13. Climate action Galactose Food Science |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Tagatose is a rare sugar with increasing commercial interest as sweetener. Biotechnological production of d-tagatose by enzymatic isomerization of d-galactose provides an alternative to chemical processes. In the last years, l-arabinose isomerases (L-AIs) from different origins have been studied to increase the effectiveness of tagatose production. In this work, the L-AI from Bacillus subtilis, previously reported to have unique substrate specificity for l-arabinose, was expressed in Escherichia coli and studied for isomerization of d-galactose to d-tagatose. The recombinant enzyme demonstrated, for the first time, tagatose bioconversion capacity, reaching ~59% conversion. Furthermore, a sustainable tagatose production strategy was developed by using Gelidium sesquipedale red seaweed and its undervalued processing residues as source of galactose. L-AI successfully converted the galactose-rich hydrolysate, obtained from direct acid hydrolysis of seaweed, to tagatose (50.9% conversion). Additionally, the process combining autohydrolysis of G. sesquipedale and acid hydrolysis of the remaining residue allowed a full integral valorisation of polysaccharides: 13.33 g of agar, an important hydrocolloid, coupled with the production of 5.97 g of tagatose. These results confirmed that seaweed biomass and waste-derived are promising substrates for tagatose production by L-AI, contributing to the advancement of circular economy and to the actual needs of food industry. This study was supported by the Portuguese Foundation for Science and Technology (FCT, Portugal) under the scope of the strategic funding of UIDB/04469/2020, the PhD grant (SFRH/BD/132717/2017 to SLB), and Biomass and Bioenergy Research Infrastructure (BBRI)- LISBOA-01- 0145-FEDER-022059 funded by the European Regional Development Fund (ERDF) under the scope of Norte2020 - Programa Operacional Regional do Norte info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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