Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system
| Autor: | Weiwei Ren, Yanqing Ren, Cairui Yang, Bing Xue, Xinhua Li, Xiumei Cheng, Di Wang, Huimin Fang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Endothelin(ET) medicine.medical_specialty lcsh:QH471-489 Uterus Antigens CD34 Estrous Cycle Ovary lcsh:Gynecology and obstetrics Nitric oxide Microcirculation Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine medicine Animals lcsh:Reproduction Testosterone Receptor Progesterone lcsh:RG1-991 Estrous cycle Estradiol Receptors Endothelin Research Cold-Shock Response Endothelins Endothelin receptor Obstetrics and Gynecology Actins 030104 developmental biology medicine.anatomical_structure Reproductive Medicine chemistry Female Cold stress 030217 neurology & neurosurgery Developmental Biology Hormone |
| Zdroj: | Reproductive Biology and Endocrinology, Vol 18, Iss 1, Pp 1-13 (2020) Reproductive Biology and Endocrinology : RB&E |
| ISSN: | 1477-7827 |
| DOI: | 10.1186/s12958-020-00584-1 |
| Popis: | Background Cold, an environmental factor, induces many reproductive diseases. It is known that endothelin (ET) is a potent vasoconstrictor, and cold stress can increase the expression of ET and its receptors. The cold stress rat model was developed to examine two parameters: (1) the effects of cold stress on ovarian and uterine morphology, function, and microvascular circulation and (2) possible mechanisms of ET and its receptors involved in cold stress-induced menstruation disorders. Methods The rat cold stress model was prepared with an ice water bath. The estrous cycle was observed by methylene blue and hematoxylin and eosin (H&E) staining. Serum estradiol 2 (E2), testosterone (T), progesterone (P) were detected by radioimmunoassay. Hemorheology indices were measured. The real-time blood flow of auricle and uterine surfaces was measured. Expressions of CD34 and α-SMA in ovarian and uterine tissues were detected by immunohistochemistry. ET-1 contents in serum were tested, and expressions of ET-receptor types A and B (ET-AR and ET-BR) in ovarian tissues were detected via Western blotting. Results Cold stress extended the estrous cycle, thereby causing reproductive hormone disorder, imbalance of local endothelin/nitric oxide expression, and microcirculation disturbance. Cold-stress led to up-regulation of ET-AR expression and protein and down-regulation of ET-BR expression in rats. Conclusions This study suggests that the reason for cold stress-induced dysfunction in reproductive organs may be closely related to the imbalance of ET-1 and its receptor expressions, leading to microvascular circulation disorders in local tissues. |
| Databáze: | OpenAIRE |
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