Transcriptomic analysis of incised leaf-shape determination in birch
| Autor: | Jing Jiang, Guifeng Liu, Lin Lin, Huai-Zhi Mu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Biology
Models Biological DNA sequencing Transcriptome Tissue culture Auxin Gene Expression Regulation Plant Botany Genetics Hexadecyltrimethylammonium bromide Betula chemistry.chemical_classification Gene ontology Sequence Analysis RNA Gene Expression Profiling fungi food and beverages Molecular Sequence Annotation General Medicine Pathway analysis Plant Leaves Gene Ontology chemistry Betula pendula Metabolic Networks and Pathways |
| Zdroj: | Gene. 531(2) |
| ISSN: | 1879-0038 |
| Popis: | Plant researchers have focused much attention on leaf shape because of its importance in the identification. To evaluate the impact of intraspecies leaf-shape variation on the transcriptome, a series of Betula pendula ‘Dalecarlica’ and B. pendula saplings were generated through tissue culture. The leaf shapes and transcriptomes of B. pendula ‘Dalecarlica’ clones were compared with those of B. pendula clones. The leaf shape of B. pendula ‘Dalecarlica’ was incised and that of B. pendula was ovate. Transcriptome data revealed numerous changes in gene expression between B. pendula ‘Dalecarlica’ and B. pendula, including upregulation of 8767 unigenes and downregulation of 8379 unigenes in B. pendula ‘Dalecarlica’. A pathway analysis revealed that the transport and signal transduction of auxin were altered in ‘Dalecarlica’, which may have contributed to its altered leaf shape. These results shed light on variation in birch leaf shape and help identify important genes for the genetic engineering of birch trees. |
| Databáze: | OpenAIRE |
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