Heterogenous mismatch-repair status in colorectal cancer
| Autor: | Mef Nilbert, Louise Laurberg Klarskov, Eva Rambech, Anders Bojesen, Patrick Joost, Maria Harbo, Nynke Veurink, Susanne Holck, Bo Baldetorp |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
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Pathology Base Pair Mismatch Denmark PMS2 Promoter Regions Genetic Mismatch Repair Endonuclease PMS2 Adenosine Triphosphatases Aged 80 and over MLH1 Nuclear Proteins General Medicine Middle Aged Immunohistochemistry DNA-Binding Proteins MutS Homolog 2 Protein Tumor Markers Biological DNA mismatch repair Microsatellite Instability MutL Protein Homolog 1 Colorectal Neoplasms Adult medicine.medical_specialty Histology Biology Pathology and Forensic Medicine Mismatch repair Biomarkers Tumor medicine Humans Adaptor Proteins Signal Transducing Aged Sweden Research Microsatellite instability Mismatch Repair Protein DNA Methylation MSH6 medicine.disease MSH2 DNA Repair Enzymes Cancer and Oncology Cancer research heterogeneity |
| Zdroj: | Joost, P, Veurink, N, Holck, S, Klarskov, L, Bojesen, A, Harbo, M, Baldetorp, B, Rambech, E & Nilbert, M 2014, ' Heterogenous mismatch-repair status in colorectal cancer ', Diagnostic Pathology, vol. 9, 126, pp. 126 . https://doi.org/10.1186/1746-1596-9-126 Diagnostic Pathology; 9, no 126 (2014) Diagnostic Pathology |
| ISSN: | 1746-1596 |
| DOI: | 10.1186/1746-1596-9-126 |
| Popis: | Background Immunohistochemical staining for mismatch repair proteins is efficient and widely used to identify mismatch repair defective tumors. The tumors typically show uniform and widespread loss of MMR protein staining. We identified and characterized colorectal cancers with alternative, heterogenous mismatch repair protein staining in order to delineate expression patterns and underlying mechanisms. Methods Heterogenous staining patterns that affected at least one of the mismatch repair proteins MLH1, PMS2, MSH2 and MSH6 were identified in 14 colorectal cancers. Based on alternative expression patterns macro-dissected and micro-dissected tumor areas were separately analyzed for microsatellite instability and MLH1 promoter methylation. Results Heterogenous retained/lost mismatch repair protein expression could be classified as intraglandular (within or in-between glandular formations), clonal (in whole glands or groups of glands) and compartmental (in larger tumor areas/compartments or in between different tumor blocks). These patterns coexisted in 9/14 tumors and in the majority of the tumors correlated with differences in microsatellite instability/MLH1 methylation status. Conclusions Heterogenous mismatch repair status can be demonstrated in colorectal cancer. Though rare, attention to this phenomenon is recommended since it corresponds to differences in mismatch repair status that are relevant for correct classification. Virtual Slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1771940323126788 |
| Databáze: | OpenAIRE |
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