Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8
| Autor: | Peter L. Bergquist, K M Borges |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Restriction Mapping Microbiology Genome Restriction fragment Restriction map Cloning Molecular Deoxyribonucleases Type II Site-Specific Molecular Biology Genome size Genetics biology Thermus Thermus thermophilus Nucleic Acid Hybridization biology.organism_classification Molecular biology Electrophoresis Gel Pulsed-Field Molecular Weight Restriction enzyme Genes Bacterial NdeI Multigene Family biology.protein bacteria Genome Bacterial Research Article |
| Popis: | A physical map of the chromosome of the extremely thermophilic eubacterium Thermus thermophilus HB8 has been constructed by using pulsed-field gel electrophoresis techniques. A total of 26 cleavage sites for the rarely cutting restriction endonucleases HpaI, MunI, and NdeI were located on the genome. On the basis of the sizes of the restriction fragments generated, the genome size was estimated to be 1.74 Mbp, which is significantly smaller than the chromosomes of Escherichia coli and other mesophiles. Partial digestion experiments revealed the order of the six HpaI bands on the chromosome. Hybridization of isolated restriction fragments to pulsed-field gel-separated restriction digestions confirmed the deduced order of the HpaI fragments and allowed ordering and alignment of the NdeI and MunI fragments. In addition, 16 genes or gene clusters cloned from several different Thermus strains were located on the T. thermophilus HB8 chromosomal map by hybridization of gene probes to pulsed-field gel-resolved restriction digestions. |
| Databáze: | OpenAIRE |
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