Implication of the nucleus in excitation contraction coupling of heart cells
| Autor: | Pierre Pothier, Ghassan Bkaily, Nadine Gros-Louis, Doris Jaalouk, Radha Naik |
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| Rok vydání: | 1996 |
| Předmět: |
Time Factors
Fura-2 Clinical Biochemistry Analytical chemistry chemistry.chemical_element Chick Embryo Calcium Biology law.invention chemistry.chemical_compound Confocal microscopy law medicine Myocyte Animals Molecular Biology Cells Cultured Fluorescent Dyes Cell Nucleus Fluo-3 Aniline Compounds Microscopy Confocal Myocardium Heart Cell Biology General Medicine Myocardial Contraction Cytosol medicine.anatomical_structure Spectrometry Fluorescence chemistry Xanthenes Cytoplasm Biophysics Nucleus |
| Zdroj: | Molecular and cellular biochemistry. 154(2) |
| ISSN: | 0300-8177 |
| Popis: | In the present study, Fluo-3 Ca2+ measurement and confocal microscopy techniques were used in order to localize cytosolic []c and nuclear []n free Ca2+ distribution in resting and spontaneously contracting single heart cells from 10-day-old chick embryos. In resting single cells, the concentration of Ca2+ in the cytoplasm was lower than that in the nucleus. Increasing cytosolic free Ca2+ from 100-1600 nM gradually increased [Ca2+]n with a maximum capacity near 1200 nM. Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes. Calcium waves in spontaneously contracting cells were found to spread from one cell to the next with the nucleus acting as a fluorescent beacon in which Ca2+ levels remained elevated for several milliseconds even after cytosolic Ca2+ had returned to near basal values. These results strongly suggest that the nucleus plays a negative and positive feedback role in controlling cytosolic free Ca2+ concentration during excitation-contraction coupling in heart cells. |
| Databáze: | OpenAIRE |
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