Ferritin Genes Overexpression in PBMC and a Rise in Exercise Performance as an Adaptive Response to Ischaemic Preconditioning in Young Men

Autor: Andzelika Borkowska, Jedrzej Antosiewicz, Agata Grzybkowska, Katarzyna Anczykowska, Piotr Sawicki, Małgorzata Żychowska, Jan Mieszkowski, Andrzej Kochanowicz, Magdalena Kochanowicz, Bartłomiej Niespodziński
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Adult
Male
Volunteers
Article Subject
Adolescent
lcsh:Medicine
Transferrin receptor
030204 cardiovascular system & hematology
Peripheral blood mononuclear cell
General Biochemistry
Genetics and Molecular Biology
Andrology
03 medical and health sciences
Young Adult
0302 clinical medicine
Antigens
CD
Receptors
Transferrin
Medicine
Humans
RNA
Messenger
cardiovascular diseases
Exercise physiology
Ischemic Preconditioning
Exercise
Wingate test
Cross-Over Studies
General Immunology and Microbiology
biology
business.industry
lcsh:R
General Medicine
Crossover study
Adaptation
Physiological
Ferritin
030104 developmental biology
Real-time polymerase chain reaction
Gene Expression Regulation
Apoferritins
Ferritins
biology.protein
Leukocytes
Mononuclear
business
Anaerobic exercise
Research Article
Zdroj: BioMed Research International, Vol 2019 (2019)
BioMed Research International
ISSN: 2314-6141
2314-6133
Popis: Objectives. The proposal of this study was to evaluate the effect of acute and ten-day ischaemic preconditioning (IPC) training procedure on the Wingate Anaerobic Test (WAnT), the ferritin H (FTH), ferritin L (FTL), and transferrin receptor 1 (TFRC) mRNA expression in peripheral blood mononuclear cells (PBMC), and anaerobic performance.Method. 34 healthy men volunteers (aged 20.7 ± 1.22 years) participated in the study. The effects of bilateral upper limb IPC and sham controlled condition were assessed in two experimental protocols: (a) the influence of acute (one time) IPC based on an experimental crossover study design and (b) the influence of ten-day IPC training treatment based on a random group assignment. At the beginning and at the end of each experiment upper body WAnT was performed and blood samples were collected to assess gene expression via quantitative PCR (qPCR).Results. No significant effect of one-time ischaemic preconditioning procedure was observed on upper body WAnT performance. Ten-day IPC training significantly increased upper limbs relative mean power (from 5.29 ± 0.50 to 5.79 ± 0.70 (W/kg), p<0.05). One-time IPC caused significant decrease inFTH,FTL, andTFRCmRNA levels while 10 days of IPC resulted in significant increase ofFTHandFTLmRNA (from 2 ∧254.2 to 2 ∧1678.6 (p = 0.01) forFTHand 2 ∧81.5 to 2 ∧923 (p = 0.01) forFTL) and decrease inTFRCmRNA.Conclusions. Our findings suggest that ten-day IPC training intervention significantly affects upper limb relative peak power. The observed overexpression of FTH and FTL genes could be associated with adaptation response induced by prolonged IPC.
Databáze: OpenAIRE
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