Single nucleotide polymorphism genotyping and distribution of Coxiella burnetii strains from field samples in Belgium
| Autor: | Ludovic Martinelle, Guy Czaplicki, Robert Renaville, Christine Thys, L. Delooz, Bénédicte Renaville, Fabiana Dal Pozzo, Nathalie Kirschvink, Fabien Grégoire, Claude Saegerman, François Smeets |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine Genotype 030106 microbiology Cattle Diseases Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Applied Microbiology and Biotechnology 03 medical and health sciences Belgium Animals Cattle Coxiella burnetii Goat Diseases Goats Humans Phylogeny Q Fever Biotechnology Food Science Ecology SNP Bulk tank Polymorphism Pathogen Genotyping Public and Environmental Health Microbiology business.industry Single Nucleotide biology.organism_classification Air sample business |
| Zdroj: | Applied and Environmental Microbiology. 82(1):81-86 |
| ISSN: | 0099-2240 |
| Popis: | The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen. |
| Databáze: | OpenAIRE |
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