Quantitative Trace Analysis of Complex Mixtures Using SABRE Hyperpolarization

Autor:	Nan Eshuis, Marco Tessari, Martin C. Feiters, Bram J. A. van Weerdenburg, Sybren S. Wijmenga, Floris P. J. T. Rutjes
Rok vydání:	2014
Předmět:	Spins Chemistry Analytical chemistry General Chemistry Nuclear magnetic resonance spectroscopy Synthetic Organic Chemistry General Medicine Small molecule Catalysis Spectral line Molecule Trace analysis Hyperpolarization (physics) Biophysical Chemistry Signal amplification
Zdroj:	Angewandte Chemie. International Edition, 54, 5, pp. 1481-1484 Angewandte Chemie. International Edition, 54, 1481-1484
ISSN:	0044-8249 1433-7851
DOI:	10.1002/ange.201409795
Popis:	Signal amplification by reversible exchange (SABRE) is an emerging nuclear spin hyperpolarization technique that strongly enhances NMR signals of small molecules in solution. However, such signal enhancements have never been exploited for concentration determination, as the efficiency of SABRE can strongly vary between different substrates or even between nuclear spins in the same molecule. The first application of SABRE for the quantitative analysis of a complex mixture is now reported. Despite the inherent complexity of the system under investigation, which involves thousands of competing binding equilibria, analytes at concentrations in the low micromolar range could be quantified from single-scan SABRE spectra using a standard-addition approach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e8649fb189f7fc3fe2af81a0cdecbb9 https://doi.org/10.1002/ange.201409795 Zobrazit plný text záznamu Plný text