Leydig Cells in Immunocastrated Polish Landrace Pig Testis: Differentiation Status and Steroid Enzyme Expression Status
| Autor: | Piotr Pawlicki, Anna Galuszka, Laura Pardyak, Ryszard Tuz, Bartosz J. Płachno, Martyna Malopolska, Klaudia Dubniewicz, Ping Yang, Malgorzata Kotula-Balak, Kazimierz Tarasiuk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
boar GnRH immunocastration Leydig cell steroidogenic enzymes relaxin-like proteins endocrine system Receptor Platelet-Derived Growth Factor alpha Swine Catalysis Gonadotropin-Releasing Hormone Inorganic Chemistry Aromatase Testis Animals Testosterone Physical and Theoretical Chemistry Molecular Biology Spectroscopy Estradiol urogenital system Organic Chemistry Leydig Cells General Medicine relaxin‐like proteins Computer Science Applications Steroids Poland |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 6120 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23116120 |
| Popis: | Porker immunocastration against gonadoliberin (GnRH) secretion has been utilized since 2009; however, consumers are still skeptical of it. This is due to not having full information available on the problem of a boar taint, as well as a lack of research on morphological and molecular changes that may occur in the animal reproductive system and other body systems. The present study aimed to explore the functional status of steroidogenic Leydig cells of the testicular interstitial tissue in immunocastrated Polish Landrace pigs. Analyses were performed using Western blot, immunohistochemistry for relaxin (RLN), insulin-like 3 protein (INSL3), pelleted growth factor receptor α (PDGFRα), cytochrome P450scc, 3β- and 17β-hydroxysteroid dehydrogenases (3β-HSD, 17β-HSD), cytochrome P450arom, and 5α-reductase (5α-RED). Immunoassay ELISA was used to measure the androstenone, testosterone, and estradiol levels in the testis and serum of immunocastrates. We revealed disturbances in the distribution and expression of (i) RLN, indicating an inflammatory reaction in the interstitial tissue; (ii) INSL3 and PDGFRα, indicating alterations in the differentiation and function of fetal, perinatal, or adult Leydig cell populations; (iii) P450scc, 3β-HSD, 17β-HSD, P450arom, and 5α-RED, indicating disturbances in the sex steroid hormone production and disturbed functional status of Leydig cells; as well as (iv) decreased levels of androstenone, testosterone, and estradiol in testicular tissue and serum, indicating the dedicated action of Improvac to reduce boar taint at both the hypothalamic–hypophysis–gonadal axis and local level (Leydig cells). In summary, our study provides a significant portion of knowledge on the function of Leydig cells after immunocastration, which is also important for the diagnosis and therapy of testis dysfunction due to GnRH action failure and/or Leydig cell differentiational–functional alterations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |