A role for early oral exposure to house dust mite allergens through breast milk in IgE-mediated food allergy susceptibility

Autor: Samara Rabelo Medeiros, Debra J. Palmer, Jessica Metcalfe, Samantha Zanelli, Nicolas Halloin, Susan L. Prescott, Patricia Macchiaverni, Valérie Verhasselt, Chrystelle Bonnart, Meri K. Tulic, Jon Genuneit, Akila Rekima, Samah Rekima
Přispěvatelé: School of Molecular Sciences, The University of Western Australia (UWA), Institut National de la Santé et de la Recherche Médicale (INSERM), Telethon KIDS Institute, Université de Nice Sophia-Antipolis (UNSA), Worldwide Universities Network, Partenaires INRAE, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire LTEE, Universidade Federal de Minas Gerais, University of Western Australia, ProdInra, Migration, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
Rok vydání: 2020
Předmět:
CD4-Positive T-Lymphocytes
Male
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Dermatophagoides pteronyssinus
Administration
Oral
0302 clinical medicine
Pregnancy
Lactation
Intestine
Small
Immunology and Allergy
2. Zero hunger
Mice
Inbred BALB C
biology
Innate lymphoid cell
3. Good health
Cysteine Endopeptidases
Milk
medicine.anatomical_structure
Female
Disease Susceptibility
Adult
Ovalbumin
Immunology
Mice
Transgenic
Breast milk
Arthropod Proteins
House dust mite
03 medical and health sciences
Double-Blind Method
Food allergy
medicine
Animals
Humans
Antigens
Dermatophagoides
Egg Hypersensitivity
food allergy
business.industry
Infant
Newborn
protease
Allergens
Immunoglobulin E
Interleukin-33
biology.organism_classification
medicine.disease
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
030104 developmental biology
030228 respiratory system
breast-feeding
Egg allergy
biology.protein
business
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Breast feeding
Zdroj: Journal of Allergy and Clinical Immunology
Journal of Allergy and Clinical Immunology, Elsevier, 2020, pp.1-25. ⟨10.1016/j.jaci.2019.12.912⟩
Journal of Allergy and Clinical Immunology, 2020, pp.1-25. ⟨10.1016/j.jaci.2019.12.912⟩
ISSN: 0091-6749
Popis: International audience; Background: Successful prevention of food allergy requires the identification of the factors adversely affecting the capacity to develop oral tolerance to food antigen in early life. Objectives: This study sought to determine whether oral exposure to Dermatophagoides pteronyssinus through breast milk affects gut mucosal immunity with long-term effects on IgE-mediated food allergy susceptibility. Methods: Gut immunity was explored in 2-week-old mice breast-fed by mothers exposed to D pteronyssinus, protease-inactivated D pteronyssinus, or to PBS during lactation. We further analyzed oral tolerance to a bystander food allergen, ovalbumin (OVA). In a proof-of-concept study, Der p 1 and OVA levels were determined in 100 human breast milk samples and the association with prevalence of IgE-mediated egg allergy at 1 year was assessed. Results: Increased permeability, IL-33 levels, type 2 innate lymphoid cell activation, and TH2 cell differentiation were found in gut mucosa of mice nursed by mothers exposed to D pteronyssinus compared with PBS. This pro-TH2 gut mucosal environment inhibited the induction of antigen-specific FoxP3 regulatory T cells and the prevention of food allergy by OVA exposure through breast milk. In contrast, protease-inactivated D pteronyssinus had no effect on offspring gut mucosal immunity. Based on the presence of Der p 1 and/or OVA in human breast milk, we identified groups of lactating mothers, which mirror the ones found in mice to be responsible for different egg allergy risk. Conclusions: This study highlights an unpredicted potential risk factor for the development of food allergy, that is, D pteronyssinus allergens in breast milk, which disrupt gut immune homeostasis and prevents oral tolerance induction to bystander food antigen through their protease activity. © 2020 American Academy of Allergy, Asthma & Immunology
Databáze: OpenAIRE
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