Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies
| Autor: | Kelen C. R. Malmegrim, Dimas Tadeu Covas, Juçara Gastaldi Cominal, Kamilla Swiech, Amanda Mizukami, Carolina Caliari-Oliveira, Jose Lucas M. Rocha, N. C. Nadia de Cassia Noronha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Stromal cell Pro-inflammatory cytokines Mesenchymal stromal cells Medicine (miscellaneous) Priming (immunology) Review Biochemistry Genetics and Molecular Biology (miscellaneous) Cell therapy Proinflammatory cytokine Biomaterials lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine In vivo Medicine lcsh:QD415-436 FÁRMACOS lcsh:R5-920 business.industry Mesenchymal stem cell Correction Cell Biology Pharmaceutical drugs 030104 developmental biology Priming 030220 oncology & carcinogenesis Cancer research Molecular Medicine Stem cell Culture conditions business lcsh:Medicine (General) Ex vivo |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Stem Cell Research & Therapy Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-21 (2019) |
| Popis: | Multipotent mesenchymal stromal cells (MSC) have been widely explored for cell-based therapy of immune-mediated, inflammatory, and degenerative diseases, due to their immunosuppressive, immunomodulatory, and regenerative potentials. Preclinical studies and clinical trials have demonstrated promising therapeutic results although these have been somewhat limited. Aspects such as low in vivo MSC survival in inhospitable disease microenvironments, requirements for ex vivo cell overexpansion prior to infusions, intrinsic differences between MSC and different sources and donors, variability of culturing protocols, and potency assays to evaluate MSC products have been described as limitations in the field. In recent years, priming approaches to empower MSC have been investigated, thereby generating cellular products with improved potential for different clinical applications. Herein, we review the current priming approaches that aim to increase MSC therapeutic efficacy. Priming with cytokines and growth factors, hypoxia, pharmacological drugs, biomaterials, and different culture conditions, as well as other diverse molecules, are revised from current and future perspectives. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|