Two problems in antisense biotechnology: in vitro delivery and the design of antisense experiments
| Autor: | C.A Stein |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Intracellular Fluid
Cell type Endosome media_common.quotation_subject Biophysics Biology Biochemistry Drug Delivery Systems Downregulation and upregulation Structural Biology Gene expression Genetics Animals Humans Internalization media_common Drug Carriers Oligonucleotide Membrane Proteins Oligonucleotides Antisense Thionucleotides Molecular biology In vitro Cell Compartmentation Cell biology Gene Expression Regulation Research Design Antisense oligonucleotides Biotechnology |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1489:45-52 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(99)00143-8 |
| Popis: | Antisense oligonucleotides are invaluable reagents for the specific downregulation of gene expression. In the absence of a carrier, charged oligonucleotides (e.g., phosphorothioates) can interact with a large number of cell surface proteins, but tend to be internalized into the endosomal/lysosomal compartment. However, they can be successfully delivered to the nuclei of diverse cell types via the use of a wide variety of reagents, including cationic lipids, and cationic polyamines. Over the past several years, a more general understanding of the rules governing the interpretation of data derived from antisense experiments has been reached. These are discussed with emphasis on how to avoid some of the confounding features of this important, emerging technology. |
| Databáze: | OpenAIRE |
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