Detection of bacteria in bovine ovarian follicular fluid
| Autor: | Arman Salary, Mojtaba Kafi, Abdollah Derakhshandeh, Maryam Sadat Moezzi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Coagulase
Lipopolysaccharides 0106 biological sciences Staphylococcus aureus endocrine system Sterility Uterus Cattle Diseases medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences 010608 biotechnology Follicular phase Escherichia coli medicine Animals Escherichia coli Infections 0303 health sciences biology 030306 microbiology Staphylococcal Infections biology.organism_classification Follicular fluid Follicular Fluid Milk medicine.anatomical_structure Cattle Female Staphylococcus Bacteria |
| Zdroj: | Letters in Applied Microbiology. 70:137-142 |
| ISSN: | 1472-765X 0266-8254 |
| DOI: | 10.1111/lam.13254 |
| Popis: | The aim of this study was to examine the sterility of follicular fluid in large ovarian follicles in dairy cows. In all, 17 samples of paired follicular fluids and uterine contents collected from post-slaughtered dairy cows were cultured to detect aerobic and facultative anaerobic bacteria. Furthermore, the origin of the bacterial isolates from samples of follicular fluid and the uteri was also investigated using PFGE analysis. Follicular fluid concentrations of lipopolysaccharides were also determined. Of 17 uterine samples, 15 (88%) were detected as contaminated. In total, nine different bacterial genera and species were identified in the uterine and follicular fluid samples. Escherichia coli was the most prevalent bacterial species isolated from the uterine samples. Out of seven isolates of Staphylococcus aureus from the uterine samples, 6 (85%) were coagulase positive. Six isolates of Staphylococcus spp. were identified in 6 out of 17 follicular fluid samples (35%). Two out of six isolates were identified as Staphylococcus aureus (33%). Our results show that ovarian follicular fluid is not sterile in the bovine. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows. SIGNIFICANCE AND IMPACT OF THE STUDY: The results of the present study show that ovarian follicular fluid is not sterile in bovines. The presence of Staphylococcus aureus in follicular fluid may partly explain the occurrence of infertility in some dairy cows. |
| Databáze: | OpenAIRE |
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