Enhanced antioxidant defense due to extracellular catalase activity in Syrian hamster during arousal from hibernation
| Autor: | Shigeharu Fukuda, Tsunetaka Ohta, Hitomi Ohta, Shigeyuki Arai, Toshiharu Hanaya, Iwao Okamoto |
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| Rok vydání: | 2006 |
| Předmět: |
Hibernation
medicine.medical_specialty Antioxidant Physiology Health Toxicology and Mutagenesis medicine.medical_treatment Hamster Toxicology medicine.disease_cause PC12 Cells Biochemistry Antioxidants chemistry.chemical_compound Cricetinae Internal medicine medicine Animals chemistry.chemical_classification Glutathione Peroxidase Mesocricetus biology Glutathione peroxidase Hydrogen Peroxide Cell Biology General Medicine Glutathione Catalase biology.organism_classification Rats Glutathione Reductase Endocrinology chemistry biology.protein Female Arousal Oxidative stress |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 143:484-491 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2006.05.002 |
| Popis: | Mammalian hibernators are considered a natural model for resistance to ischemia-reperfusion injuries, and protective mechanisms against oxidative stress evoked by repeated hibernation-arousal cycles in these animals are increasingly the focus of experimental investigation. Here we show that extracellular catalase activity provides protection against oxidative stress during arousal from hibernation in Syrian hamster. To examine the serum antioxidant defense system, we first assessed the hibernation-arousal state-dependent change in serum attenuation of cytotoxicity induced by hydrogen peroxide. Serum obtained from hamsters during arousal from hibernation at a rectal temperature of 32 degrees C, concomitant with the period of increased oxidative stress, attenuated the cytotoxicity four-fold more effectively than serum from cenothermic control hamsters. Serum catalase activity significantly increased during arousal, whereas glutathione peroxidase activity decreased by 50%, compared with cenothermic controls. The cytoprotective effect of purified catalase at the concentration found in serum was also confirmed in a hydrogen peroxide-induced cytotoxicity model. Moreover, inhibition of catalase by aminotriazole led to an 80% loss of serum hydrogen peroxide scavenging activity. These results suggest that extracellular catalase is effective for protecting hibernators from oxidative stress evoked by arousal from hibernation. |
| Databáze: | OpenAIRE |
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