Role of mast cell chymase in allergen-induced biphasic skin reaction
| Autor: | Harukazu Fukami, Namino Sugiura, Tsuyoshi Muto, Yoshiaki Fukuda, Motoo Sumida, Nobuo Tsuruoka, Kayo Saito, Masayuki Saito, Chika Horikawa, Yoshiaki Tomimori, Saki Kakutani, Kyoko Yamashiro |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Allergy Histamine Antagonists Inflammation Pharmacology Biochemistry Dermatitis Atopic Mice chemistry.chemical_compound Histamine receptor Chymases Internal medicine Hypersensitivity Leukocytes medicine Animals Edema Humans Mast Cells Intradermal injection Mice Inbred BALB C integumentary system Chemotaxis Serine Endopeptidases Chymase medicine.disease Mast cell Recombinant Proteins Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Mechanism of action medicine.symptom Histamine |
| Zdroj: | Biochemical Pharmacology. 64:1187-1193 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/s0006-2952(02)01265-0 |
| Popis: | Intradermal injection of human chymase (EC 3.4.21.39) into the mouse ear elicited an edematous skin reaction in a biphasic manner, with a transient reaction peaking at 1 hr, followed by a delayed response persisting for at least 24 hr. The kinetics of this reaction was analogous to the biphasic skin reaction induced by ascaris extract in actively sensitized mice. A similarity between the two dermatitis models was also shown by histological analysis, i.e. accumulation of inflammatory cells was observed exclusively in the later phases of the skin reaction. A chymase inhibitor, SUN-C8077 [3-(3-aminophenylsulfonyl)-7-chloroquinazorine 2,4(1 H , 3 H )-dione], significantly inhibited both the early- and late-phase responses of the skin reaction induced by ascaris extract. These findings suggest that chymase may play an important role in the allergen-induced biphasic skin reaction. A histamine receptor antagonist, homochlorcyclizine, inhibited the early-phase but not the late-phase of the chymase-induced skin reaction. In addition, human chymase showed chemotactic activity to human polymorphonuclear leukocytes in vitro . Mast cell chymase may participate in the two phases of allergic skin inflammation by two distinct mechanisms, i.e. histamine- and leukocyte-dependent mechanisms, respectively. |
| Databáze: | OpenAIRE |
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