Degradation of alicyclic molecules by Rhodococcus ruber CD4
| Autor: | R. M. Fakoussa, J. D. Schumacher |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Oxygenase Cyclododecane Ketone biology Molecular mass Flavoprotein General Medicine Applied Microbiology and Biotechnology Ring size Alicyclic compound chemistry.chemical_compound Biodegradation Environmental chemistry Hydrocarbons Alicyclic biology.protein Oxygenases Organic chemistry Rhodococcus Oxidation-Reduction Lactone Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 52(1) |
| ISSN: | 0175-7598 |
| Popis: | The present work describes investigations on the bacterial degradation of the alicyclic molecule cyclododecane. It represents a structure where the initial degradative steps have to be similar to a "subterminal" attack as there is no "terminal" part of the molecule. We were able to show that the gram-positive bacterium Rhodococcus ruber CD4 DSM 44394 oxidizes cyclododecane to the corresponding alcohol and ketone, the latter being subject to ring fission by a Baeyer-Villiger oxygenase. This key enzyme is an NADPH- and O2-dependent flavoprotein with a substrate specificity for bigger rings. The further metabolism of the resulting lactone gives rise to an omega-hydroxyalkanoic acid that is susceptible to common beta-oxidation. Due to its alicyclic character and its ring size, cyclododecane is comparable to aliphatic bridge components that are an important element in the coal texture. They contribute to the three-dimensional coal structure and thus could serve as a valuable target for the oxidative abilities of R. ruber CD4 to reduce the molecular mass of coal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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