Analysis of differentially expressed genes in nitric oxide-exposed human monocytic cells
| Autor: | Alexandre Diet, Jean-Claude Drapier, Kyril Turpaev, Cécile Bouton, Annie Glatigny |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Molybdopterin synthase Biology Alkenes Nitric Oxide Biochemistry Monocytes Cyclic N-Oxides Transcription (biology) Physiology (medical) Gene expression Humans Nitric Oxide Donors Northern blot RNA Messenger Gene Transcription factor Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Imidazoles Free Radical Scavengers Blotting Northern Molecular biology Real-time polymerase chain reaction Gene Expression Regulation Gene chip analysis Biomarkers |
| Zdroj: | Free radical biologymedicine. 38(10) |
| ISSN: | 0891-5849 |
| Popis: | In this study we examined the gene expression pattern of *NO-dependent genes in U937 and Mono Mac 6 monocytes exposed to the synthetic NO-donor DPTA-NO using microarray technology. cDNA microarray data were validated by Northern blot analysis and quantitative real-time PCR. This approach allowed the identification of 17 *NO-sensitive genes that showed at least a twofold difference in expression, in both U937 cells and Mono Mac 6 cells exposed to 500 microM DPTA-NO for 4 h. NO-stimulated genes belong to various functional groups, including transcription factors, signaling molecules, and cytokines. Among the selected genes, 11 (ATF-4, c-maf, SGK-1, PBEF, ATPase 8, NADH dehydrogenase 4, STK6, TRAF4-associated factor 1, molybdopterin synthase, CKS1, and CIDE-B) have not been previously reported to be sensitive to *NO. Because several *NO-stimulated genes are transcription factors, we analyzed the mRNA expression profile in U937 cells exposed to DPTA-NO for 14 h. We found that long-term *NO treatment influenced transcription rates of a rather limited set of genes, including CIDE-B, BNIP3, p21/Cip1, molybdopterin synthase, and TRAF4-associated factor 1. To accelerate formation of nitrosating species, U937 cells were exposed to DPTA-NO along with suboptimal concentrations of 2-phenyl-4,4,5,5-tetramethylimidazole-1-oxyl 3-oxide (PTIO). PTIO-mediated increase in nitrosating species remarkably enhanced *NO-dependent induction of IL-8, p21/Cip1, and MKP-1 and built a specific gene expression profile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect