Genetic subtypes of HIV-1 in the Philippines
| Autor: | Ofelia T. Monzon, Gerald H. Learn, Hideaki Tsuchie, Takashi Kurimura, Mari Rose A. Aplasca, Fem Julia E. Paladin |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Sexually transmitted disease Philippines Molecular Sequence Data Immunology Population HIV Infections Genome Viral Biology Polymerase Chain Reaction Acquired immunodeficiency syndrome (AIDS) medicine Humans Immunology and Allergy Amino Acid Sequence Genetic variability Child education Phylogeny Subtypes of HIV education.field_of_study Phylogenetic tree Molecular epidemiology Transmission (medicine) Infant Sequence Analysis DNA Middle Aged medicine.disease Virology Infectious Diseases DNA Viral HIV-1 Female Sequence Alignment |
| Zdroj: | AIDS. 12:291-300 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/00002030-199803000-00007 |
| Popis: | Although HIV-1 infection reached Asia late relative to other regions and countries the degree of HIV infection climbed to epidemic proportions over the past 5 years. In 1995 2.5 million of the 4.7 million new HIV infections worldwide occurred in southeast Asia. The Philippines National HIV Sentinel Surveillance Program operational since 1993 has estimated that the current HIV prevalence among a representative population of male and female prostitutes men having sex with men IV drug users and male sexually transmitted disease patients is about 1%. The transmission pattern among 71% of reported cases follows that of a typical heterosexually transmitted disease. Findings are reported from a study conducted to determine the genetic variability of HIV-1 among infected Filipinos and to analyze phylogenetic relationships temporal introductions and transmission dynamics of identified variants. Findings are based upon polymerase chain reaction amplification and the direct sequencing of a 204 base-pair fragment of the env C2-V3 region from uncultured peripheral blood mononuclear cells obtained from 51 HIV-1-positive Filipinos infected between 1987 and mid-1996. The evolutionary distance and phylogenetic relationships among the DNA sequences were estimated. The strains were classified as follows: 37 subtype B 8 subtype E 3 subtype A 2 subtype C and 1 subtype D. The overall env nucleotide divergence ranged from 11.7% to 32.2% while the nucleotide variation appeared to be random and no temporal ordering was observed. Variation of the sequences at the tip of the V3 loop was very broad. Subtypes B and C isolates showed no close genetic relationship to other Asian variants and only 3 of the subtype E strains had close affinity to known Asian sequences. About 66% of cases are thought to have been infected outside of the Philippines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|