POLAR-guided signalling complex assembly and localization drive asymmetric cell division
| Autor: | Daniel Valentin Savatin, Miroslava Zhiponova, Anaxi Houbaert, Camilla Betti, Alberto de Marcos Serrano, Isabelle Vanhoutte, Sjef Boeren, Carmen Fenoll, Sacco C. de Vries, Manish Tiwari, Mounashree J. Urs, Geert De Jaeger, Thomas Jacobs, Mansour Karimi, Kun Wang, Gustavo Eduardo Gudesblat, Cheng Zhang, Dominique Eeckhout, Eugenia Russinova, Montaña Mena |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Scaffold protein MAP Kinase Signaling System Arabidopsis Biochemie Cell Cycle Proteins macromolecular substances Biochemistry Substrate Specificity Ciencias Biológicas Glycogen Synthase Kinase 3 03 medical and health sciences Cytosol GSK Biología Celular Microbiología Cell polarity Basic Helix-Loop-Helix Transcription Factors Asymmetric cell division Life Science Cell Lineage Phosphorylation Protein kinase A Transcription factor Stomata Multidisciplinary Arabidopsis Proteins Kinase Chemistry Asymmetric Cell Division Speechless Cell Polarity Cell biology Phenotype 030104 developmental biology Multiprotein Complexes Plant Stomata Brassinosteroid Signal transduction Protein Kinases CIENCIAS NATURALES Y EXACTAS Protein Binding Signal Transduction |
| Zdroj: | Nature, 563(7732), 574-578 Nature 563 (2018) 7732 |
| ISSN: | 0028-0836 |
| Popis: | Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants. Fil: Houbaert, Anaxi. University of Ghent; Bélgica Fil: Zhang, Cheng. University of Ghent; Bélgica Fil: Tiwari, Manish. University of Ghent; Bélgica Fil: Wang, Kun. Wuhan University; China. University of Ghent; Bélgica Fil: Marcos Serrano, Alberto de. Universidad de Castilla-La Mancha; España Fil: Savatin, Daniel V.. University of Ghent; Bélgica Fil: Urs, Mounashree J.. University of Ghent; Bélgica Fil: Zhiponova, Miroslava. University of Sofia; Bulgaria. University of Ghent; Bélgica Fil: Gudesblat, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. University of Ghent; Bélgica Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica Fil: Eeckhout, Dominique. University of Ghent; Bélgica Fil: Boeren, Sjef. Wageningen University; Países Bajos Fil: Karimi, Mansour. University of Ghent; Bélgica Fil: Betti, Camilla. University of Ghent; Bélgica. Università di Perugia; Italia Fil: Jacobs, Thomas. University of Ghent; Bélgica Fil: Fenoll, Carmen. Universidad de Castilla-La Mancha; España Fil: Mena, Montaña. Universidad de Castilla-La Mancha; España Fil: de Vries, Sacco. Wageningen University; Países Bajos Fil: De Jaeger, Geert. University of Ghent; Bélgica Fil: Russinova, Eugenia. University of Ghent; Bélgica |
| Databáze: | OpenAIRE |
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