In vitro anti-leishmanial activity of Prunus armeniaca fractions on Leishmania tropica and molecular docking studies

Autor: Aneeqa Hamid, Naveeda Akhter Qureshi, Nargis Shaheen, Attiya Iqbal, Asma Ashraf, Huma Fatima
Rok vydání: 2020
Předmět:
Male
Antioxidant
Leishmania tropica
Prunus armeniaca
medicine.medical_treatment
Carboxylic acid
030303 biophysics
Biophysics
Ethyl acetate
Antiprotozoal Agents
Carboxylic Acids
Drug Evaluation
Preclinical
02 engineering and technology
Aldehyde
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
chemistry.chemical_compound
Nitric acid
Alkanes
medicine
Benzene Derivatives
Animals
Humans
Radiology
Nuclear Medicine and imaging
Carbazic acid
Sulfhydryl Compounds
chemistry.chemical_classification
0303 health sciences
Aldehydes
Mice
Inbred BALB C
Radiation
Radiological and Ultrasound Technology
biology
Plant Extracts
021001 nanoscience & nanotechnology
biology.organism_classification
Thin-layer chromatography
Molecular Docking Simulation
Plant Leaves
Hydrazines
chemistry
Alkynes
0210 nano-technology
Cyclobutanes
Nuclear chemistry
Zdroj: Journal of photochemistry and photobiology. B, Biology. 213
ISSN: 1873-2682
Popis: Prunus armeniaca (L.) is a member of the Rosaceae, subfamily Prunoideae, shows anticancer, antitubercular, antimutagenic, antimicrobial, antioxidant, and cardioprotective activities. Here we fractionated the leaves extract of this highly medicinally important plant for antileishmanial activity. In the current study, the leaves extract was fractionated and characterized using column and thin layer chromatography by n-hexane, ethyl acetate, and methanol solvents. Twelve fractions were isolated and subjected for evaluation of their cytotoxicity and in vitro antileishmanial activity against promastigotes and amastigotes of Leishmania tropica. Among all fractions used, the fraction (F7) exhibited the strongest antileishmanial activity. The bioactive fraction was further characterized by spectroscopy (FTIR, UV–Vis), and GC-MS analysis. The in silico docking was carried out to find the active site of PTR1. All derived fractions exhibited toxicity in the safety range IC50 > 100 μg/ml. The fraction (F7) showed significantly the highest antipromastigotes activity with IC5011.48 ± 0.82 μg/ml and antiamastigotes activity with IC50 21.03 ± 0.98 μg/ml compared with control i.e. 11.60 ± 0.70 and 22.03 ± 1.02 μg/ml respectively. The UV–Vis spectroscopic analysis revealed the presence of six absorption peaks and the FTIR spectrum revealed the presence of alkane, aldehyde, carboxylic acid, thiols, alkynes, and carbonyls compounds The GC–MS chromatogram exhibited the presence of nine compounds: (a) benzeneethanol, alpha, beta dimethyl, (b)carbazic acid, 3-(1 propylbutylidene)-, ethyl ester, (c)1, 2-benzenedicarboxylic acid, diisooctyl ester, (d)benzeneethanamine a-methyl, (e)2aminononadecane, (f)2-heptanamine-5-methyl, (g)cyclobutanol, (h)cyclopropyl carbine, and (i)nitric acid, nonyl ester. Among all compounds, the 1, 2-benzenedicarboxylic acid, diisooctyl ester bound well to the PTR1 receptor. Fraction (F7) showed acceptable results with no cytotoxicity. However, in vivo studies are required in the future.
Databáze: OpenAIRE
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