Unexpected expression of alpha- and beta-globin in mesencephalic dopaminergic neurons and glial cells
| Autor: | Roberto Simone, Marta Biagioli, Daniela Cesselli, Vittorio Gallo, Elio Raviola, Charles Plessy, Nicolas Bertin, Dejan Lazarevic, Claudio Santoro, Marta Zaninello, Christina Vlachouli, Antonio Paolo Beltrami, Isidro Ferrer, Stefano Rivella, Paola Roncaglia, Kazuto Kobayashi, Milena F. Pinto, Stefano Gustincich, Carlo Alberto Beltrami, Piero Carninci |
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| Přispěvatelé: | Biagioli, M, Pinto, M, Cesselli, D, Zaninello, M, Lazarevic, D, Roncaglia, P, Simone, R, Vlachouli, Christina, Plessy, C, Bertin, N, Beltrami, A, Kobayashi, K, Gallo, V, Santoro, C, Ferrer, I, Rivella, S, Beltrami, Ca, Carninci, P, Raviola, E, Gustincich, S., Universitat de Barcelona |
| Rok vydání: | 2009 |
| Předmět: |
Green Fluorescent Proteins
oxidative phosphorylation Nigrostriatal pathway oligodendrocytes Citologia Substantia nigra Neurones beta-Globins Biology Hippocampal formation Mice A9 neurons alpha-Globins alpha and beta globins Dopaminergic Cell medicine Animals Humans Globin Parkinson Oligonucleotide Array Sequence Analysis astrocytes hemoglobin Neurons Multidisciplinary dopaminergic cell Ventral Tegmental Area Dopaminergic Parkinson Disease Anatomy Biological Sciences A9 neuron Flow Cytometry Metabolisme Rats Cell biology Substantia Nigra Ventral tegmental area microarray medicine.anatomical_structure Metabolism Neuroglia Neuròglia Cytology alpha and beta globin |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | The mesencephalic dopaminergic (mDA) cell system is composed of two major groups of projecting cells in the substantia nigra (SN) (A9 neurons) and the ventral tegmental area (VTA) (A10 cells). A9 neurons form the nigrostriatal pathway and are involved in regulating voluntary movements and postural reflexes. Their selective degeneration leads to Parkinson's disease. Here, we report that gene expression analysis of A9 dopaminergic neurons (DA) identifies transcripts for α- and β-chains of hemoglobin (Hb). Globin immunoreactivity decorates the majority of A9 DA, a subpopulation of cortical and hippocampal astrocytes and mature oligodendrocytes. This pattern of expression was confirmed in different mouse strains and in rat and human. We show that Hb is expressed in the SN of human postmortem brain. By microarray analysis of dopaminergic cell lines overexpressing α- and β-globin chains, changes in genes involved in O 2 homeostasis and oxidative phopshorylation were observed, linking Hb expression to mitochondrial function. Our data suggest that the most famed oxygen-carrying globin is not exclusively restricted to the blood, but it may play a role in the normal physiology of the brain and neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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