Alginate/poloxamer hydrogel obtained by thiol-acrylate photopolymerization for the alleviation of the inflammatory response of human keratinocytes
| Autor: | Raluca Nicoleta Darie-Nita, Irina Popescu, Maria Anghelache, Maria Valentina Dinu, Dana Mihaela Suflet, Mihaela Turtoi, Marieta Constantin, Manuela Calin |
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| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
Light Alginates Cell Survival Anti-Inflammatory Agents Poloxamer 02 engineering and technology Acryloyl chloride Biochemistry Polymerization 03 medical and health sciences chemistry.chemical_compound Structural Biology HaCaT Cells Humans Sulfhydryl Compounds Molecular Biology Cell Proliferation 030304 developmental biology Wound Healing 0303 health sciences Acrylate Swelling capacity Hydrogels General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Elasticity Cross-Linking Reagents Photopolymer Acrylates chemistry Self-healing hydrogels Biophysics Cysteamine 0210 nano-technology Wound healing Porosity Bandages Hydrocolloid |
| Zdroj: | International Journal of Biological Macromolecules. 180:418-431 |
| ISSN: | 0141-8130 |
| Popis: | Hydrogel-based wound dressings have been intensively studied as promising materials for wound healing and care. The mixed-mode thiol-acrylate photopolymerization is used in this paper for alginate/poloxamer hydrogels formation. First, the alginate was modified with thiol groups using the esterification reaction with cysteamine, and second, the terminal hydroxyl groups of poloxamer were esterified with acryloyl chloride to introduce polymerizable acrylate groups. Finally, the cross-linking reaction between the two macromers was performed to produce degradable alginate/poloxamer hydrogels. The optimum conditions for the photo-initiated reaction were studied in order to obtain high gel fractions. The resulting hydrogels have high swelling capacity in simulated physiological conditions, good elasticity and strength, and appropriate porosity, some of the physico-chemical properties required for their applications as wound dressings/patches. The biological assays show that the alginate/poloxamer hydrogels induce proliferation of human keratinocyte and have an anti-inflammatory effect on lipopolysaccharides (LPS)-activated keratinocytes by inhibiting the extracellular signal-regulated kinases (ERK)/ nuclear factor (NF)-kB/ tumor necrosis factor (TNF)-α signalling pathway. Taken together, the results showed that the chemical cross-linked alginate/poloxamer hydrogels may function as a dressing/patch applied directly on the skin lesion to heal the wound by reducing the exacerbated inflammation, the main cause of wound healing delay and local infection. |
| Databáze: | OpenAIRE |
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