Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription
| Autor: | Françoise Stutz, Manuel S. Rodriguez, Batool Ossareh-Nazari, Catherine Dargemont, Maria Hobeika, Gilles Divita, Nahid Iglesias, Carole Gwizdek |
|---|---|
| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Department of Cell Biology, Sciences III, University of Geneva [Switzerland], Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), IJM (UMR_7592) - Institut Jacques Monod, Centre National de la Recherche Scientifique (CNRS)-IFR122-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
RNA
Messenger/genetics Nucleocytoplasmic Transport Proteins Proteasome Endopeptidase Complex Saccharomyces cerevisiae Proteins Transcription Genetic THO complex [SDV]Life Sciences [q-bio] RNA-binding protein Saccharomyces cerevisiae [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Saccharomyces cerevisiae Proteins/genetics/metabolism DNA-binding protein 03 medical and health sciences Ubiquitin ddc:570 Nuclear Proteins/genetics/metabolism RNA Messenger RNA-Binding Proteins/genetics/metabolism Nuclear protein Nuclear export signal ComputingMilieux_MISCELLANEOUS Transcription Genetic/genetics 030304 developmental biology Ribonucleoprotein 0303 health sciences Messenger RNA DNA-Binding Proteins/metabolism Multidisciplinary biology Nucleocytoplasmic Transport Proteins/genetics/metabolism 030302 biochemistry & molecular biology Nuclear Proteins RNA-Binding Proteins Biological Transport Biological Sciences Molecular biology DNA-Binding Proteins Proteasome Endopeptidase Complex/metabolism biology.protein Ubiquitin/metabolism Saccharomyces cerevisiae/genetics/metabolism Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (44), pp.16376-81. ⟨10.1073/pnas.0607941103⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (44), pp.16376-16381. ⟨10.1073/pnas.0607941103⟩ Proceedings of the National Academy of Sciences, Vol. 103, No 44 (2006) pp. 16376-81 |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.0607941103⟩ |
| Popis: | The mRNA nuclear export receptor Mex67/Mtr2 is recruited to mRNAs through RNA-binding adaptors, including components of the THO/TREX complex that couple transcription to mRNA export. Here we show that the ubiquitin-associated (UBA) domain of Mex67 is not only required for proper nuclear export of mRNA but also contributes to recruitment of Mex67 to transcribing genes. Our results reveal that the UBA domain of Mex67 directly interacts with polyubiquitin chains and with Hpr1, a component of the THO/TREX complex, which is regulated by ubiquitylation in a transcription-dependent manner. This interaction transiently protects Hpr1 from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|