GintABC1 encodes a putative ABC transporter of the MRP subfamily induced by Cu, Cd, and oxidative stress in Glomus intraradices
| Autor: | Concepción Azcón-Aguilar, Karim Benabdellah, Ascensión Valderas, Manuel González-Guerrero, Nuria Ferrol |
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| Rok vydání: | 2009 |
| Předmět: |
Models
Molecular Paraquat Subfamily DNA Complementary Molecular Sequence Data ATP-binding cassette transporter Plant Science Biology Models Biological Gene Expression Regulation Enzymologic Fungal Proteins Complementary DNA Gene Expression Regulation Fungal Gene expression Genetics Transcriptional regulation Amino Acid Sequence DNA Fungal Glomeromycota Molecular Biology Peptide sequence Ecology Evolution Behavior and Systematics Phylogeny Fungal protein Sequence Homology Amino Acid fungi Computational Biology Transporter General Medicine Sequence Analysis DNA Oxidative Stress Biochemistry ATP-Binding Cassette Transporters Copper Cadmium |
| Zdroj: | Mycorrhiza. 20(2) |
| ISSN: | 1432-1890 |
| Popis: | A full-length cDNA sequence putatively encoding an ATP-binding cassette (ABC) transporter (GintABC1) was isolated from the extraradical mycelia of the arbuscular mycorrhizal fungus Glomus intraradices. Bioinformatic analysis of the sequence indicated that GintABC1 encodes a 1513 amino acid polypeptide, containing two six-transmembrane clusters (TMD) intercalated with sequences characteristics of the nucleotide binding domains (NBD) and an extra N-terminus extension (TMD0). GintABC1 presents a predicted TMD0-(TMD-NBD)(2) topology, typical of the multidrug resistance-associated protein subfamily of ABC transporters. Gene expression analyses revealed no difference in the expression levels of GintABC1 in the extra- vs the intraradical mycelia. GintABC1 was up-regulated by Cd and Cu, but not by Zn, suggesting that this transporter might be involved in Cu and Cd detoxification. Paraquat, an oxidative agent, also induced the transcription of GintABC1. These data suggest that redox changes may be involved in the transcriptional regulation of GintABC1 by Cd and Cu. |
| Databáze: | OpenAIRE |
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