Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA

Autor: Liana S. Mkrtchyan, Ludmila I. Krikunova, Selivanova Ei, Irina A. Zamulaeva, Kiseleva Vi, Matchuk On
Rok vydání: 2021
Předmět:
cancer stem cells
cervical cancer
medicine.medical_treatment
Uterine Cervical Neoplasms
Alphapapillomavirus
lcsh:Chemistry
Medicine
human papillomavirus
lcsh:QH301-705.5
Lymph node
Spectroscopy
Cervical cancer
medicine.diagnostic_test
biology
General Medicine
Middle Aged
Prognosis
Computer Science Applications
Hyaluronan Receptors
medicine.anatomical_structure
Carcinoma
Squamous Cell
Neoplastic Stem Cells
Regression Analysis
Female
Stem cell
Adult
Real-Time Polymerase Chain Reaction
Article
Catalysis
Flow cytometry
Inorganic Chemistry
Young Adult
Cancer stem cell
Humans
Physical and Theoretical Chemistry
Molecular Biology
radiotherapy
Aged
business.industry
flow cytometry
Papillomavirus Infections
Organic Chemistry
CD44
CD24 Antigen
medicine.disease
Radiation therapy
lcsh:Biology (General)
lcsh:QD1-999
Drug Resistance
Neoplasm
DNA
Viral
Cancer research
biology.protein
business
Chemoradiotherapy
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 3
International Journal of Molecular Sciences, Vol 22, Iss 1445, p 1445 (2021)
ISSN: 1422-0067
Popis: Radio- and chemoresistance of cancer stem cells (CSCs) is considered as one of the possible causes of adverse results of chemoradiotherapy for various malignancies, including cervical cancer. However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and mechanisms for individual response of CSCs to therapy. The purpose of the study was to evaluate the association of radiation response of cervical CSCs with clinical and morphological parameters of disease and features of human papillomavirus (HPV) infection. The proportion of CD44+CD24low CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86, p = 0.001 for the multiple regression model in the whole).
Databáze: OpenAIRE
Externí odkaz: