Impact of neural cell adhesion molecule deletion on regeneration after mouse spinal cord injury
| Autor: | Melitta Schachner, Gabriele Loers, Igor Jakovcevski, Vedangana Saini, Gurcharan Kaur |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Nervous system medicine.medical_specialty Genotype metabolism [Axons] Biology metabolism [Spinal Cord Injuries] Mice 03 medical and health sciences 0302 clinical medicine Injury Site Cell Movement Internal medicine medicine genetics [Neural Cell Adhesion Molecules] Animals ddc:610 Neural Cell Adhesion Molecules Spinal cord injury Cells Cultured Spinal Cord Injuries physiology [Axons] metabolism [Astrocytes] Glial fibrillary acidic protein genetics [Spinal Cord Injuries] General Neuroscience physiology [Astrocytes] medicine.disease Spinal cord Axons Nerve Regeneration Mice Inbred C57BL metabolism [Neural Cell Adhesion Molecules] 030104 developmental biology medicine.anatomical_structure Endocrinology nervous system Astrocytes biology.protein Female Neural cell adhesion molecule Neuroscience Locomotion 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | European journal of neuroscience 44(1), 1734-1746 (2016). doi:10.1111/ejn.13271 |
| ISSN: | 0953-816X |
| DOI: | 10.1111/ejn.13271 |
| Popis: | The neural cell adhesion molecule (NCAM) plays important functional roles in development of the nervous system. We investigated the influence of a constitutive ablation of NCAM on the outcome of spinal cord injury. Transgenic mice lacking NCAM (NCAM-/-) were subjected to severe compression injury of the lower thoracic spinal cord using wild-type (NCAM+/+) littermates as controls. According to the single-frame motion analysis, the NCAM-/- mice showed reduced locomotor recovery in comparison to control mice at 3 and 6 weeks after injury, indicating an overall positive impact of NCAM on recovery after injury. Also the Basso Mouse Scale score was lower in NCAM-/- mice at 3 weeks after injury, whereas at 6 weeks after injury the difference between genotypes was not statistically significant. Worse locomotor function was associated with decreased monoaminergic and cholinergic innervation of the spinal cord caudal to the injury site and decreased axonal regrowth/sprouting at the site of injury. Astrocytic scar formation at the injury site, as assessed by immunohistology for glial fibrillary acidic protein at and around the lesion site was increased in NCAM-/- compared with NCAM+/+ mice. Migration of cultured monolayer astrocytes from NCAM-/- mice was reduced as assayed by scratch wounding. Numbers of Iba-1 immunopositive microglia were not different between genotypes. We conclude that constitutive NCAM deletion in young adult mice reduces recovery after spinal cord injury, validating the hypothesized beneficial role of this molecule in recovery after injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|