Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
| Autor: | Yang Pan, Li Sha Wei, Guang Ming Yang, Tao Tao, Jie Sun, Min-Sheng Zhu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Vascular smooth muscle Contraction (grammar) General Chemical Engineering Pharmacology General Biochemistry Genetics and Molecular Biology Contractility Mice 03 medical and health sciences Isometric Contraction medicine Animals Mesenteric arteries General Immunology and Microbiology Electrical impedance myography Chemistry General Neuroscience Myography Smooth muscle contraction Mesenteric Arteries 030104 developmental biology medicine.anatomical_structure Medicine Vascular smooth muscle contraction Myograph |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58064 |
| Popis: | The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K(+) solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K(+) solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K(+) solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening. |
| Databáze: | OpenAIRE |
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