In vitro exposure effects of cyclosporin A and bis(tri-n-butyltin)oxide on lymphocyte proliferation, cytokine (receptor) mRNA expression, and cell surface marker expression in rat thymocytes and splenocytes
| Autor: | H. van Loveren, Sander W. Spiekstra, Rob J. Vandebriel, Clive Meredith, B. N. Hudspith |
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| Přispěvatelé: | Dermatology |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Cell Survival
Lymphocyte medicine.medical_treatment CD8 Antigens Gene Expression Lymphocyte proliferation Thymus Gland Biology Toxicology Lymphocyte Activation Polymerase Chain Reaction Flow cytometry Interferon-gamma Antigens CD Cyclosporin a medicine Animals Lymphocytes RNA Messenger Rats Wistar medicine.diagnostic_test Dose-Response Relationship Drug Nucleic Acid Hybridization Receptors Interleukin-2 Molecular biology Interleukin-10 Rats Thymocyte medicine.anatomical_structure Cytokine Apoptosis CD4 Antigens Cyclosporine Cytokines Interleukin-2 Female Trialkyltin Compounds Cytokine receptor Cell Division Immunosuppressive Agents Spleen |
| Zdroj: | Vandebriel, RJ, Spiekstra, SW, Hudspith, BN, Meredith, C & Van Loveren, H 1999, ' In vitro exposure effects of cyclosporin A and bis(tri-n-butyltin)oxide on lymphocyte proliferation, cytokine (receptor) mRNA expression, and cell surface marker expression in rat thymocytes and splenocytes ', Toxicology, vol. 135, no. 1, pp. 49-66 . Toxicology, 135(1), 49-66. Elsevier Ireland Ltd |
| ISSN: | 0300-483X |
| Popis: | Rat thymocytes and splenocytes were exposed in vitro to the model compounds Cyclosporin A (CsA), an immunosuppressive drug, and bis(tri-n-butyltin)oxide (TBTO), an immunotoxic environmental contaminant. The lymphocyte transformation test (LTT), cytokine (receptor) mRNA expression (RT-PCR and dot blot hybridisation), and flow cytometry were evaluated as assays for in vitro immunotoxicity, at dose levels that did not show effects on viability, this being the aim of the study. LTT and RT-PCR proved useful assays. Lymphocyte transformation was suppressed by both compounds, while IL-2 mRNA expression was suppressed by CsA but not by TBTO, and both compounds suppressed IL-2R mRNA expression in splenocytes but not in thymocytes. Furthermore, the data obtained suggest that antiproliferative effects may be more relevant than apoptosis induction for TBTO induced thymus atrophy. |
| Databáze: | OpenAIRE |
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