GSK3 is a multifunctional regulator ofDictyosteliumdevelopment
| Autor: | Jeffrey G. Williams, Tsuyoshi Araki, Adrian J. Harwood, Hazel P. Williams, Christina Schilde |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Slug Cellular differentiation Molecular Sequence Data Spores Protozoan Mutant Population Protozoan Proteins Glycogen Synthase Kinase 3 Cyclic AMP Animals Gene family Dictyostelium Amino Acid Sequence education Molecular Biology Genetics Regulation of gene expression Extracellular Matrix Proteins education.field_of_study biology Gene Expression Profiling Gene Expression Regulation Developmental Cell Differentiation biology.organism_classification Clone Cells Agar STAT Transcription Factors Multicellular organism Mutation Sequence Alignment Biomarkers Gene Deletion Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Development. 131:4555-4565 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.01330 |
| Popis: | Glycogen synthase kinase 3 (GSK3) is a central regulator of metazoan development and the Dictyostelium GSK3 homologue, GskA, also controls cellular differentiation. The originally derived gskA-null mutant exhibits a severe pattern formation defect. It forms very large numbers of pre-basal disc cells at the expense of the prespore population. This defect arises early during multicellular development, making it impossible to examine later functions of GskA. We report the analysis of a gskA-null mutant, generated in a different parental strain, that proceeds through development to form mature fruiting bodies. In this strain, Ax2/gskA–,early development is accelerated and slug migration greatly curtailed. In a monolayer assay of stalk cell formation, the Ax2/gskA– strain is hypersensitive to the stalk cell-inducing action of DIF-1 but largely refractory to the repressive effect exerted by extracellular cAMP. During normal development, apically situated prestalk cells express the ecmBgene just as they commit themselves to stalk cell differentiation. In the Ax2/gskA– mutant, ecmB is expressed throughout the prestalk region of the slug, suggesting that GskA forms part of the repressive signalling pathway that prevents premature commitment to stalk cell differentiation. GskA may also play an inductive developmental role, because microarray analysis identifies a large gene family, the 2C family, that require gskA for optimal expression. These observations show that GskA functions throughout Dictyostelium development, to regulate several key aspects of cellular patterning. |
| Databáze: | OpenAIRE |
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