Effect of agitation speed and aeration rate on fructosyltransferase production of Aspergillus oryzae IPT-301 in stirred tank bioreactor
| Autor: | Maria Filomena de Andrade Rodrigues, Rafael Firmani Perna, Alfredo Eduardo Maiorano, Cristiane Angélica Ottoni, Beatriz Guilarte Maresma, Elda Sabino da Silva, Rubén Cuervo Fernandez, Rosane A. M. Piccoli |
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| Přispěvatelé: | Institute for Technological Research (IPT), Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Federal University of Alfenas (ICT/UNIFAL-MG), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Food industry Aspergillus oryzae Oligosaccharides Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Bioreactors KLa Biotransformation 010608 biotechnology Fructooligosaccharides β-fructofuranosidase Bioreactor Extracellular Food science biology business.industry General Medicine biology.organism_classification Enzyme production Carbon Culture Media Aspergillus 030104 developmental biology Hexosyltransferases chemistry Fermentation Fructosyltransferase Aeration business Biotechnology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-020-03006-9 |
| Popis: | Made available in DSpace on 2021-06-25T10:11:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes–fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. Results: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g−1 and 667 U dm−3 h−1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm−3. The higher value of total activity obtained was 22,831 U dm−3 at 0.75 vvm and 800 rpm. Conclusion: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production. Bionanomanufacturing Center Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532 Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Vía Blanca 804, Carretera Central, San Miguel del Padrón Institute of Science and Technology Federal University of Alfenas (ICT/UNIFAL-MG), José Aurélio Vilela Road, 11999 BR 267 Km 533 Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/no Instituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178 Biosciences Institute São Paulo State University (UNESP), Praça Infante Dom Henrique s/no Instituto de Estudos Avançados do Mar (IEAMar) São Paulo State University, Av. João Frascisco Bensdorp 1178 |
| Databáze: | OpenAIRE |
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