Insulin inhibits its own secretion from isolated, perifused human pancreatic islets
| Autor: | E. Finke, B. J. Olack, P. E. Lacy, D. W. Scharp, C. J. Swanson, Rosa Giannarelli, M. Mclear, Piero Marchetti, Renzo Navalesi |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Time Factors Endocrinology Diabetes and Metabolism medicine.medical_treatment Biology Feedback Islets of Langerhans chemistry.chemical_compound Endocrinology Internal medicine Insulin Secretion Internal Medicine medicine Humans Insulin Incubation Cells Cultured Pancreatic hormone geography geography.geographical_feature_category C-Peptide Dose-Response Relationship Drug C-peptide Pancreatic islets General Medicine Islet Perfusion Kinetics Glucose medicine.anatomical_structure L-Glucose chemistry Basal (medicine) |
| Zdroj: | Acta Diabetologica. 32:75-77 |
| ISSN: | 1432-5233 0940-5429 |
| DOI: | 10.1007/bf00569560 |
| Popis: | It is still a controversial question whether insulin suppresses its own secretion. We prepared pure human islets from three pancreases by collagenase digestion and density gradient purification. Aliquots of 200 islet equivalents (IE, 150-μm sized-islets) were sequentially perifused at 37°C with 3.3 mmol/l glucose (3.3G, 40 min), 16.7 mmol/l glucose (16.7G, 30 min) and again 3.3G (30 min) after 24 h, 37°C culture in CMRL 1066 medium with or without the addition of either 200 or 400 μU/ml human insulin in the incubation medium (6 replicates each). Insulin secretion was assessed by C-peptide (Cp) measurement in the perufusate. Without added insulin (C) and with 200 (Ins200) or 400 (Ins400) μU/ml added insulin, basal Cp release was 0.12±0.03, 0.14±0.02 and 0.14±0.04 ng/ml, respectively. At 16.7G, the first-phase secretion peak (expressed as Cp value) was significantly lower with Ins200 (0.47±0.13 ng/ml,P |
| Databáze: | OpenAIRE |
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