Reduction of phase thickness is a characteristic reaction of the nucleoli to toxicity as shown by coherent phase microscopy
| Autor: | P. S. Ignat’yev, T. V. Vyshenskaya, V. P. Tychinskii, N. T. Raikhlin, A. B. Ivanov, Alexander A. Shtil, A. V. Kretushev, N. A. Filippova, I. V. Klemyashov |
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| Rok vydání: | 2007 |
| Předmět: |
Depolymerization
Nucleolus Phase contrast microscopy General Medicine Biology Microtubules General Biochemistry Genetics and Molecular Biology law.invention Cell biology chemistry.chemical_compound Adenosine Triphosphate Microscopy Electron Transmission chemistry Microtubule law Cell Line Tumor Toxicity Humans Microscopy Phase-Contrast Electron microscope Adenosine triphosphate Cell Nucleolus Intracellular |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 143:493-497 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-007-0165-0 |
| Popis: | Optical parameters of human cell nucleoli (HCT116 colorectal cancer cells) in depolymerization of microtubules and depletion of intracellular ATP pool were studied by coherent phase microscopy. These influences were associated with a rapid (recorded within the first minutes) reduction of the phase thickness of the nucleoli. These changes are similar to the nucleolar response to direct inhibitors of transcription. Hence, quantitative parameters of coherent phase microscopy describe common reaction of the nucleolus to stress; reduction of optical thickness of the nucleolus is a component of this reaction. |
| Databáze: | OpenAIRE |
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